The role of eosinophil major basic protein in angiogenesis

I. Puxeddu, N. Berkman, A. H. Nissim Ben Efraim, D. E. Davies, D. Ribatti, G. J. Gleich, F. Levi-Schaffer

Research output: Contribution to journalArticle

Abstract

Background: Eosinophil-derived major basic protein (MBP) plays an active role in allergic inflammation and tissue remodelling. However, its role in angiogenesis has not been established as yet. Therefore our objective was to investigate whether MBP exhibits any direct pro-angiogenic effects. Methods: Rat aortic endothelial cells and human umbilical vascular endothelial cells were cultured with different concentrations of MBP and their viability (Trypan blue exclusion test), proliferation (thymidine incorporation) and capillary-like structure formation (matrigel assay) were investigated in vitro. The angiogenic activity of MBP was then tested in vivo using the chick chorio allantoic membrane (CAM) assay. Results: Subcytotoxic concentrations of MBP induce endothelial cell proliferation and enhance the pro-mitogenic effect of vascular endothelial growth factor (VEGF), but do not affect their VEGF release. MBP promotes capillarogenesis by endothelial cells seeded on matrigel and sprouting formation in the CAM assay. Furthermore, we have shown that the pro-angiogenic effect of MBP is not due to its cationic charge since stimulation of the CAMs with the synthetic polycation, poly-l-arginine does not induce any angiogenic effects. Conclusions: These data demonstrate that MBP has pro-angiogenic effects in vitro and in vivo, providing a novel mechanism whereby MBP can participate in tissue inflammation and remodelling in atopic diseases.

Original languageEnglish
Pages (from-to)368-374
Number of pages7
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume64
Issue number3
DOIs
Publication statusPublished - Mar 2009

Keywords

  • Allergic inflammation
  • Angiogenesis
  • Eosinophils
  • Major basic protein
  • Tissue remodelling

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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  • Cite this

    Puxeddu, I., Berkman, N., Nissim Ben Efraim, A. H., Davies, D. E., Ribatti, D., Gleich, G. J., & Levi-Schaffer, F. (2009). The role of eosinophil major basic protein in angiogenesis. Allergy: European Journal of Allergy and Clinical Immunology, 64(3), 368-374. https://doi.org/10.1111/j.1398-9995.2008.01822.x