The role of interleukin-3 in classical Hodgkin's disease

Donatella Aldinucci, Karin Olivo, Debora Lorenzon, Dalisa Poletto, Annunziata Gloghini, Antonino Carbone, Antonio Pinto

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Classical Hodgkin's disease (HD) is a peculiar form of lymphoma characterized by a low frequency of tumor cells, the so-called Hodgkin (H) and Reed/Sternberg (RS) cells, embedded in a background of non-neoplastic (reactive) cells believed to be recruited and activated by H-RS cell-derived cytokines/chemokines. How these tumor cells can survive in such a seemingly hostile environment has confused researchers. We have previously identified interleukin (IL)-3 receptor (R) expression as a common feature of classical HD and unveiled the potential role of IL-3 as a growth and anti-apoptotic factor for H-RS cells. More then 90% of malignant cells of classical HD usually express the α chain of the IL-3R (IL-3Rα), as evidenced by immunostaining of frozen sections and cell suspensions from neoplastic lymph nodes. Consistently, HD-derived cell lines (L428, KMH2, HDLM2 and L1236) express the α and β chains that form IL-3R, both at the mRNA and protein level, with a molecular size of IL-3Rα identical (70 kDa) to that expressed by human myeloid cells. Exogenous IL-3 promotes the growth of cultured H-RS cells, such an effect being potentiated by IL-9 and stem cell factor (SCF) co-stimulation, and is able to partially rescue tumor cells from apoptosis induced by serum deprivation. Finally, cultured H-RS cells are able to increase the production of IL-3 by pre-activated T cells, suggesting an involvement of IL-3/IL-3R interactions in the cellular growth of HD through paracrine mechanisms. This review will outline the biological activity of IL-3 and summarize the evidence indicating IL-3 as a growth and anti-apoptotic factor for H-RS cells in classical HD.

Original languageEnglish
Pages (from-to)303-311
Number of pages9
JournalLeukemia and Lymphoma
Volume46
Issue number3
DOIs
Publication statusPublished - Mar 2005

Fingerprint

Reed-Sternberg Cells
Interleukin-3
Hodgkin Disease
Interleukins
Complement Factor H
Growth
Interleukin-3 Receptors
Interleukin-9
Neoplasms
Stem Cell Factor
Frozen Sections
Myeloid Cells
Chemokines
Lymphoma
Suspensions
Lymph Nodes
Research Personnel
Apoptosis
Cytokines
T-Lymphocytes

Keywords

  • H - RS cell growth
  • IL-3
  • IL-3 receptor
  • T cells

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

The role of interleukin-3 in classical Hodgkin's disease. / Aldinucci, Donatella; Olivo, Karin; Lorenzon, Debora; Poletto, Dalisa; Gloghini, Annunziata; Carbone, Antonino; Pinto, Antonio.

In: Leukemia and Lymphoma, Vol. 46, No. 3, 03.2005, p. 303-311.

Research output: Contribution to journalArticle

Aldinucci, Donatella ; Olivo, Karin ; Lorenzon, Debora ; Poletto, Dalisa ; Gloghini, Annunziata ; Carbone, Antonino ; Pinto, Antonio. / The role of interleukin-3 in classical Hodgkin's disease. In: Leukemia and Lymphoma. 2005 ; Vol. 46, No. 3. pp. 303-311.
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