The role of nitric oxide in focally-evoked limbic seizures

M. R. Proctor, F. Fornai, J. K B Afshar, K. Gale

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The deep rostral piriform cortex contains a site (area tempestas) in which focal application of picomole amounts of bicuculline, a GABA antagonist, triggers limbic motor seizures which are dependent upon activation of both N-methyl-D-aspartate and α-amino-3-hydroxy-5-methyloxole-4-proprionate subtypes of glutamate receptors. In the present study we determined whether nitric oxide can influence the local modulation of seizure initiation by bicuculline. Nitric oxide and the nitric oxide precursor L-arginine, alone or in combination with low doses of bicuculline were focally administered into the area tempestas of rats. While nitric oxide alone had no significant convulsant effect, L-arginine alone (30-240 nmol) induced brief myoclonic episodes. Nitric oxide (0.7 nmol) and L-arginine (30 nmol) markedly potentiated the seizures evoked by a low dose of bicuculline. The effect of L-arginine was prevented by focal pretreatment with an inhibitor of nitric oxide synthesis, N-nitro-L-arginine methyl ester. However, N-nitro-L-arginine methyl ester did not attenuate the convulsant effect of bicuculline or kainate alone when focally administered into area tempestas. The data demonstrate that exogenously applied nitric oxide or its precursors can enhance seizure triggering activity. However, the data also indicate that L-arginine-nitric oxide pathway does not normally contribute to seizure expression from area tempestas, as N-nitro-L-arginine methyl ester alone did not attenuate focally-evoked seizures.

Original languageEnglish
Pages (from-to)1231-1236
Number of pages6
JournalNeuroscience
Volume76
Issue number4
DOIs
Publication statusPublished - 1997

Fingerprint

Nitric Oxide
Seizures
Bicuculline
Arginine
Convulsants
GABA Antagonists
Kainic Acid
Glutamate Receptors
N-Methylaspartate
arginine methyl ester

Keywords

  • glutamate
  • nitric oxide
  • piriform cortex
  • seizure

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The role of nitric oxide in focally-evoked limbic seizures. / Proctor, M. R.; Fornai, F.; Afshar, J. K B; Gale, K.

In: Neuroscience, Vol. 76, No. 4, 1997, p. 1231-1236.

Research output: Contribution to journalArticle

Proctor, M. R. ; Fornai, F. ; Afshar, J. K B ; Gale, K. / The role of nitric oxide in focally-evoked limbic seizures. In: Neuroscience. 1997 ; Vol. 76, No. 4. pp. 1231-1236.
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