The role of nitrinergic connections in central cardiovascular responses mediated by physostigmine infused into posterior hypothalamus

Ernesto Palma, Carolina Muscoli, Eliana Mancuso, Francesca Sculco, Iolanda Sacco, Wanessa Alecce, Nicola Costa, Carmela Colica, Dario Cristiano, Domenicantonio Rotiroti, Vincenzo Mollace

Research output: Contribution to journalArticlepeer-review

Abstract

In the last few decades, cholinergic connections located into posterior hypothalamus (PH) have been implicated in the central regulation of blood pressure (BP). Here we investigated the role of nitric oxide (NO) in the blood pressure response elicited by infusion of physostigmine into PH of normotensive rats. In freely moving rats, physostigmine (60-200 nM) produced a dose- and time-dependent elevation of BP which was antagonized by the antimuscarinic drug scopolamine (60 nM) and by l-NAME (100 μM), an inhibitor of NO synthase, both infused into the same site. In contrast, l-arginine (l-Arg; 100 μM), the precursor of NO, and glyceryltrinitrate (GTN; 140 nM), an NO donor, infused into the PH did not affect physostigmine-related pressor response. In rats pre-treated with Escherichia coli lipopolisaccharide (LPS; 0.5 μg i.p. 24 h beforehand), however, scopolamine, l-Arg and GTN produced a decrease of BP, an effect antagonized by l-NAME. This suggests that NO only slightly modulates physostigmine-related pressor response elicited into PH of LPS-untreated rats. In contrast, the release of large amounts of NO generated by pre-treating rats with LPS, down-regulates cholinergic connections located at the PH, thus contributing in the central dysregulation of BP which can be found when high circulating endotoxin levels may occur.

Original languageEnglish
Pages (from-to)112-115
Number of pages4
JournalNeuroscience Letters
Volume368
Issue number1
DOIs
Publication statusPublished - Sep 16 2004

Keywords

  • Blood pressure
  • Cholinergic system
  • Nitric oxide
  • Posterior hypothalamus

ASJC Scopus subject areas

  • Neuroscience(all)

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