The role of prep1 in the regulation of mesenchymal stromal cells

Giorgia Maroni, Daniele Panetta, Raffaele Luongo, Indira Krishnan, Federica La Rosa, Daniela Campani, Piero Salvadori, Patricia Iozzo, Francesco Blasi, Dmitry Penkov, Elena Levantini, Maria Cristina Magli

Research output: Contribution to journalArticle

Abstract

Molecular mechanisms governing cell fate decision events in bone marrow mesenchymal stromal cells (MSC) are still poorly understood. Herein, we investigated the homeobox gene Prep1 as a candidate regulatory molecule, by adopting Prep1 hypomorphic mice as a model to investigate the effects of Prep1 downregulation, using in vitro and in vivo assays, including the innovative single cell RNA sequencing technology. Taken together, our findings indicate that low levels of Prep1 are associated to enhanced adipogenesis and a concomitant reduced osteogenesis in the bone marrow, suggesting Prep1 as a potential regulator of the adipo-osteogenic differentiation of mesenchymal stromal cells. Furthermore, our data suggest that in vivo decreased Prep1 gene dosage favors a pro-adipogenic phenotype and induces a “browning” effect in all fat tissues.

Original languageEnglish
Article number3639
JournalInternational Journal of Molecular Sciences
Volume20
Issue number15
DOIs
Publication statusPublished - Aug 1 2019

Fingerprint

bone marrow
Mesenchymal Stromal Cells
genes
Bone
Genes
Cells
osteogenesis
phenotype
sequencing
regulators
fats
Oils and fats
cells
RNA
RNA Sequence Analysis
mice
Assays
Adipogenesis
Gene Dosage
Homeobox Genes

Keywords

  • Adipogenesis
  • Homeobox gene
  • In vivo imaging
  • Mesenchymal stromal cells (MSC)
  • Murine models
  • Osteogenesis
  • Single cell RNA sequencing

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Maroni, G., Panetta, D., Luongo, R., Krishnan, I., La Rosa, F., Campani, D., ... Magli, M. C. (2019). The role of prep1 in the regulation of mesenchymal stromal cells. International Journal of Molecular Sciences, 20(15), [3639]. https://doi.org/10.3390/ijms20153639

The role of prep1 in the regulation of mesenchymal stromal cells. / Maroni, Giorgia; Panetta, Daniele; Luongo, Raffaele; Krishnan, Indira; La Rosa, Federica; Campani, Daniela; Salvadori, Piero; Iozzo, Patricia; Blasi, Francesco; Penkov, Dmitry; Levantini, Elena; Magli, Maria Cristina.

In: International Journal of Molecular Sciences, Vol. 20, No. 15, 3639, 01.08.2019.

Research output: Contribution to journalArticle

Maroni, G, Panetta, D, Luongo, R, Krishnan, I, La Rosa, F, Campani, D, Salvadori, P, Iozzo, P, Blasi, F, Penkov, D, Levantini, E & Magli, MC 2019, 'The role of prep1 in the regulation of mesenchymal stromal cells', International Journal of Molecular Sciences, vol. 20, no. 15, 3639. https://doi.org/10.3390/ijms20153639
Maroni, Giorgia ; Panetta, Daniele ; Luongo, Raffaele ; Krishnan, Indira ; La Rosa, Federica ; Campani, Daniela ; Salvadori, Piero ; Iozzo, Patricia ; Blasi, Francesco ; Penkov, Dmitry ; Levantini, Elena ; Magli, Maria Cristina. / The role of prep1 in the regulation of mesenchymal stromal cells. In: International Journal of Molecular Sciences. 2019 ; Vol. 20, No. 15.
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