The role of proopiomelanocortin-derived peptides in skin fibroblast and mast cell functions

Patrizia Teofoli, Alessandra Frezzolini, Pietro Puddu, Ornella De Pità, Alain Mauviel, Torello Lotti

Research output: Contribution to journalArticle

Abstract

We have previously described proopiomelanocortin (POMC) gene-expression in human normal cultured dermal fibroblasts, and its dose-and time-dependent modulation by transforming growth factor-α (TGF-β) and tumor necrosis factor-α (TNF-α). The aim of the work described here was to investigate POMC-derived peptide release in vitro by cultured fibroblasts following incubation with different concentrations of both TNF-α and TGF-β for 24 hours (1, 5, and 10 ng/ml). The effect of simultaneous addition of both TNF-α and TGF-β (10 ng/ml) was also evaluated. Culture supernatants of human skin fibroblasts were collected to detect adrenocorticotropin hormone (ACTH), α-melanotropin (α-MSH), and β-endorphin (β-EP) levels by specific immunoenzymatic assay. We investigated the in vitro histamine-releasing activity of the POMC-derived peptides, α-MSH and β-EP, on human foreskin mast cells. Detection of cleavage products in supernatants from cultured normal human dermal fibroblasts indicated intracellular processing by POMC protein. We were able to measure detectable levels of all peptides in basal conditions. TNF-α addition resulted in an increase in β-EP and ACTH levels, TGF-β-stimulated fibroblasts showed no alteration in β-EP and α-MSH levels, whereas ACTH release was significantly enhanced. Both α-MSH and β-EP induced histamine release from human foreskin mast cells in vitro with β-EP-induced histamine levels as high as those observed with the calcium ionophore, ionomycin. Our data document fibroblast POMC-derived peptide release and modulation by cytokines, suggesting that they have a possible role in extracellular matrix deposit regulation and skin inflammation.

Original languageEnglish
Pages (from-to)268-276
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume885
Publication statusPublished - 1999

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Pro-Opiomelanocortin
Melanocyte-Stimulating Hormones
Fibroblasts
Mast Cells
Skin
Transforming Growth Factors
Peptides
Tumor Necrosis Factor-alpha
Adrenocorticotropic Hormone
Histamine
Foreskin
Hormones
Modulation
Endorphins
Ionomycin
Calcium Ionophores
Histamine Release
Cell culture
Gene expression
Extracellular Matrix

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Teofoli, P., Frezzolini, A., Puddu, P., De Pità, O., Mauviel, A., & Lotti, T. (1999). The role of proopiomelanocortin-derived peptides in skin fibroblast and mast cell functions. Annals of the New York Academy of Sciences, 885, 268-276.

The role of proopiomelanocortin-derived peptides in skin fibroblast and mast cell functions. / Teofoli, Patrizia; Frezzolini, Alessandra; Puddu, Pietro; De Pità, Ornella; Mauviel, Alain; Lotti, Torello.

In: Annals of the New York Academy of Sciences, Vol. 885, 1999, p. 268-276.

Research output: Contribution to journalArticle

Teofoli, P, Frezzolini, A, Puddu, P, De Pità, O, Mauviel, A & Lotti, T 1999, 'The role of proopiomelanocortin-derived peptides in skin fibroblast and mast cell functions', Annals of the New York Academy of Sciences, vol. 885, pp. 268-276.
Teofoli, Patrizia ; Frezzolini, Alessandra ; Puddu, Pietro ; De Pità, Ornella ; Mauviel, Alain ; Lotti, Torello. / The role of proopiomelanocortin-derived peptides in skin fibroblast and mast cell functions. In: Annals of the New York Academy of Sciences. 1999 ; Vol. 885. pp. 268-276.
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