The role of SwrA, DegU and PD3in fla/che expression in B. subtilis

Serena Mordini, Cecilia Osera, Simone Marini, Francesco Scavone, Riccardo Bellazzi, Alessandro Galizzi, Cinzia Calvio

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

In B. subtilis swarming and robust swimming motility require the positive trigger of SwrA on fla/che operon expression. Despite having an essential and specific activity, how SwrA executes this task has remained elusive thus far. We demonstrate here that SwrA acts at the main σA-dependent fla/che promoter PA(fla/che) through DegU. Electrophoretic mobility shift assays (EMSA) reveal that SwrA forms a complex with the phosphorylated form of DegU (DegU∼P) at PA(fla/che) while it is unable to do so with either unphosphorylated DegU or the DegU32(Hy) mutant protein. Motility assays show that a highly phosphorylated DegU is not detrimental for flagellar motility provided that SwrA is present; however, DegU∼P represses P A(fla/che) in the absence of SwrA. Overall, our data support a model in which DegU∼P is a dual regulator, acting either as a repressor when alone or as a positive regulator of PA(fla/che) when combined with SwrA. Finally, we demonstrate that the σD-dependent P D3(fla/che) promoter plays an important role in motility, representing a contingent feedback loop necessary to maintain basal motility when swrA is switched to the non-functional swrA- status.

Original languageEnglish
Article numbere85065
JournalPLoS One
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 27 2013

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Electrophoretic Mobility Shift Assay
Mutant Proteins
Operon
Assays
bacterial motility
promoter regions
Electrophoretic mobility
swarming
assays
operon
Feedback
mutants
proteins
Swimming

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mordini, S., Osera, C., Marini, S., Scavone, F., Bellazzi, R., Galizzi, A., & Calvio, C. (2013). The role of SwrA, DegU and PD3in fla/che expression in B. subtilis. PLoS One, 8(12), [e85065]. https://doi.org/10.1371/journal.pone.0085065

The role of SwrA, DegU and PD3in fla/che expression in B. subtilis. / Mordini, Serena; Osera, Cecilia; Marini, Simone; Scavone, Francesco; Bellazzi, Riccardo; Galizzi, Alessandro; Calvio, Cinzia.

In: PLoS One, Vol. 8, No. 12, e85065, 27.12.2013.

Research output: Contribution to journalArticle

Mordini, S, Osera, C, Marini, S, Scavone, F, Bellazzi, R, Galizzi, A & Calvio, C 2013, 'The role of SwrA, DegU and PD3in fla/che expression in B. subtilis', PLoS One, vol. 8, no. 12, e85065. https://doi.org/10.1371/journal.pone.0085065
Mordini S, Osera C, Marini S, Scavone F, Bellazzi R, Galizzi A et al. The role of SwrA, DegU and PD3in fla/che expression in B. subtilis. PLoS One. 2013 Dec 27;8(12). e85065. https://doi.org/10.1371/journal.pone.0085065
Mordini, Serena ; Osera, Cecilia ; Marini, Simone ; Scavone, Francesco ; Bellazzi, Riccardo ; Galizzi, Alessandro ; Calvio, Cinzia. / The role of SwrA, DegU and PD3in fla/che expression in B. subtilis. In: PLoS One. 2013 ; Vol. 8, No. 12.
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