The Role of the Polo Kinase Cdc5 in Controlling Cdc14 Localization

Rosella Visintin, Frank Stegmeier, Angelika Amon

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

In budding yeast, the protein phosphatase Cdc14 controls exit from mitosis. Its activity is regulated by a competitive inhibitor Cfi1/Net1, which binds to and sequesters Cdc14 in the nucleolus. During anaphase, Cdc14 is released from its inhibitor by the action of two regulatory networks. The Cdc Fourteen Early Anaphase Release (FEAR) network initiates Cdc14 release from Cfi1/Net1 during early anaphase, and the Mitotic Exit Network (MEN) promotes Cdc14 release during late anaphase. Here, we investigate the relationship among FEAR network components and propose an order in which they function to promote Cdc14 release from the nucleolus. Furthermore, we examine the role of the protein kinase Cdc5, which is a component of both the FEAR network and the MEN, in Cdc14 release from the nucleolus. We find that overexpression of CDC5 led to Cdc14 release from the nucleolus in S phase-arrested cells, which correlated with the appearance of phosphorylated forms of Cdc14 and Cfi1/Net1. Cdc5 promotes Cdc14 phosphorylation and, by stimulating the MEN, Cfi1/Net1 phosphorylation. Furthermore, we suggest that Cdc14 release from the nucleolus only occurs when Cdc14 and Cfi1/Net1 are both phosphorylated.

Original languageEnglish
Pages (from-to)4486-4498
Number of pages13
JournalMolecular Biology of the Cell
Volume14
Issue number11
DOIs
Publication statusPublished - Nov 2003

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Anaphase
Phosphotransferases
Phosphorylation
Saccharomycetales
Phosphoprotein Phosphatases
S Phase
Mitosis
Protein Kinases

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

The Role of the Polo Kinase Cdc5 in Controlling Cdc14 Localization. / Visintin, Rosella; Stegmeier, Frank; Amon, Angelika.

In: Molecular Biology of the Cell, Vol. 14, No. 11, 11.2003, p. 4486-4498.

Research output: Contribution to journalArticle

Visintin, Rosella ; Stegmeier, Frank ; Amon, Angelika. / The Role of the Polo Kinase Cdc5 in Controlling Cdc14 Localization. In: Molecular Biology of the Cell. 2003 ; Vol. 14, No. 11. pp. 4486-4498.
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