The role of the thiol N-acetylcysteine in the prevention of tumor invasion and angiogenesis

M. Morini, T. Cai, M. G. Aluigi, D. M. Noonan, L. Masiello, S. De Flora, F. D'Agostini, A. Albini, G. Fassina

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

We have extensively studied the effects of N-acetylcysteine (NAC), a cytoprotective drug that can prevent in vivo carcinogenesis. Here we review our findings NAC completely inhibits gelatinolytic activity of metalloproteases and chemotactic and invasive activities of tumor cells. In addition, NAC reduces the number of lung metastases when malignant murine melanoma cells are injected into nude mice. NAC treatment decreases the weight of primary tumors and produces a dose-related increase in tumor latency. Moreover, oral administration of NAC reduces the formation of spontaneous metastases. In experimental metastasis assays, we have found a synergistic reduction in the number of lung metastases after treatment with doxorubicin (DOX) and NAC in nude mice. In tumorigenicity and spontaneous metastasis assays, the combined administration of DOX and oral NAC again has shown synergistic effects on the frequency and weight of primary tumors and local recurrences and completely prevented the formation of lung metastases. The addition of NAC to endothelial cells strongly reduces their invasive activity in response to angiogenic stimuli. NAC inhibited the degradation and release of radiolabeled type IV collagen by activated endothelial cells, indicating that NAC blocks gelatinase activity. Oral administration of NAC reduces the angiogenic response induced by KS tumor cell products, confirming the ability of NAC to inhibit the invasive activity of endothelial cells in vivo and thereby blocking angiogenesis.

Original languageEnglish
Pages (from-to)268-271
Number of pages4
JournalInternational Journal of Biological Markers
Volume14
Issue number4
Publication statusPublished - Oct 1999

Fingerprint

Acetylcysteine
Sulfhydryl Compounds
Tumors
Neoplasms
Neoplasm Metastasis
Endothelial cells
Oral Administration
Endothelial Cells
Tumor Burden
Nude Mice
Lung
Doxorubicin
Assays
Cells
Gelatinases
Collagen Type IV
Metalloproteases
Melanoma
Carcinogenesis

Keywords

  • Angiogenesis
  • Doxorubicin
  • Endothelial cells
  • Invasion
  • Migration
  • NAC

ASJC Scopus subject areas

  • Immunology
  • Biochemistry

Cite this

Morini, M., Cai, T., Aluigi, M. G., Noonan, D. M., Masiello, L., De Flora, S., ... Fassina, G. (1999). The role of the thiol N-acetylcysteine in the prevention of tumor invasion and angiogenesis. International Journal of Biological Markers, 14(4), 268-271.

The role of the thiol N-acetylcysteine in the prevention of tumor invasion and angiogenesis. / Morini, M.; Cai, T.; Aluigi, M. G.; Noonan, D. M.; Masiello, L.; De Flora, S.; D'Agostini, F.; Albini, A.; Fassina, G.

In: International Journal of Biological Markers, Vol. 14, No. 4, 10.1999, p. 268-271.

Research output: Contribution to journalArticle

Morini, M, Cai, T, Aluigi, MG, Noonan, DM, Masiello, L, De Flora, S, D'Agostini, F, Albini, A & Fassina, G 1999, 'The role of the thiol N-acetylcysteine in the prevention of tumor invasion and angiogenesis', International Journal of Biological Markers, vol. 14, no. 4, pp. 268-271.
Morini, M. ; Cai, T. ; Aluigi, M. G. ; Noonan, D. M. ; Masiello, L. ; De Flora, S. ; D'Agostini, F. ; Albini, A. ; Fassina, G. / The role of the thiol N-acetylcysteine in the prevention of tumor invasion and angiogenesis. In: International Journal of Biological Markers. 1999 ; Vol. 14, No. 4. pp. 268-271.
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