The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia

Francesca Granucci, Filippo Petralia, Matteo Urbano, Stefania Citterio, Francesco Di Tota, Laura Santambrogio, Paola Ricciardi-Castagnoli

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Macrophage receptor with collagenous structure (MARCO) is a scavenger receptor expressed in peritoneal macrophages and in a subpopulation of macrophages in the marginal zone of the spleen and in the medullary cord of lymph nodes. By global gene expression analysis, it has been found that the MARCO mRNA was one of the most up-regulated in splenic dendritic cells (DCs) following lipopolysaccharide or bacterial activation and in granulocyte-macrophage colony-stimulating factor (GM-CSF)-treated microglial cells. Here we show that MARCO is expressed on splenic DCs at late time points after activation and that its expression correlates with profound changes in actin cytoskeleton organization in DCs and microglia. During maturation, DCs undergo profound rearrangements of actin cytoskeleton. Immature DCs are adherent with visible actin cables, while fully mature, MARCO-expressing, splenic DCs are nonadherent, round in shape, and have an actin cytoskeleton with a punctate distribution. The simple expression of MARCO was sufficient to induce these cytoskeleton modifications in DCs. MARCO-transfected immature DCs acquired a typical morphology of mature DCs and did not rearrange the actin cytoskeleton following activation. Moreover, DCs in which MARCO was knocked down did not reach the mature phenotype and maintained the typical morphology of transitional DCs. MARCO expression in DCs and microglial cells was also associated with a decrease of antigen internalization capacity. Thus, the MARCO receptor is important for actin cytoskeleton rearrangements and the down-regulation of antigen uptake function during DC and microglial cell maturation.

Original languageEnglish
Pages (from-to)2940-2947
Number of pages8
JournalBlood
Volume102
Issue number8
DOIs
Publication statusPublished - Oct 15 2003

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Scavenger Receptors
Microglia
Cytoskeleton
Macrophages
Dendritic Cells
Actins
Actin Cytoskeleton
Chemical activation
Antigens
Peritoneal Macrophages
Granulocyte-Macrophage Colony-Stimulating Factor
Gene expression
Lipopolysaccharides

ASJC Scopus subject areas

  • Hematology

Cite this

Granucci, F., Petralia, F., Urbano, M., Citterio, S., Di Tota, F., Santambrogio, L., & Ricciardi-Castagnoli, P. (2003). The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia. Blood, 102(8), 2940-2947. https://doi.org/10.1182/blood-2002-12-3651

The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia. / Granucci, Francesca; Petralia, Filippo; Urbano, Matteo; Citterio, Stefania; Di Tota, Francesco; Santambrogio, Laura; Ricciardi-Castagnoli, Paola.

In: Blood, Vol. 102, No. 8, 15.10.2003, p. 2940-2947.

Research output: Contribution to journalArticle

Granucci, F, Petralia, F, Urbano, M, Citterio, S, Di Tota, F, Santambrogio, L & Ricciardi-Castagnoli, P 2003, 'The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia', Blood, vol. 102, no. 8, pp. 2940-2947. https://doi.org/10.1182/blood-2002-12-3651
Granucci F, Petralia F, Urbano M, Citterio S, Di Tota F, Santambrogio L et al. The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia. Blood. 2003 Oct 15;102(8):2940-2947. https://doi.org/10.1182/blood-2002-12-3651
Granucci, Francesca ; Petralia, Filippo ; Urbano, Matteo ; Citterio, Stefania ; Di Tota, Francesco ; Santambrogio, Laura ; Ricciardi-Castagnoli, Paola. / The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia. In: Blood. 2003 ; Vol. 102, No. 8. pp. 2940-2947.
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