The secretome signature of colon cancer cell lines

Esther Imperlini, Irene Colavita, Marianna Caterino, Peppino Mirabelli, Daniela Pagnozzi, Luigi Del Vecchio, Rosa Di Noto, Margherita Ruoppolo, Stefania Orrù

Research output: Contribution to journalArticle


The definition of the secretome signature of a cancer cell line can be considered a potential tool to investigate tumor aggressiveness and a preclinical exploratory study required to optimize the search of cancer biomarkers. Dealing with a cell-specific secretome limits the contamination by the major components of the human serum and reduces the range of dynamic concentrations among the secreted proteins, thus favouring under-represented tissue-specific species. The aim of the present study is to characterize the secretome of two human colon carcinoma cell lines, CaCo-2 and HCT-GEO, in order to evaluate differences and similarities of two colorectal cancer model systems. In this study, we identified more than 170 protein species, 64 more expressed in the secretome of CaCo-2 cells and 54 more expressed in the secretome of HCT-GEO cells; 58 proteins were shared by the two systems. Among them, more than 50% were deemed to be secretory according to their Gene Ontology annotation and/or to their SignalP or SecretomeP scores. Such a characterization allowed corroborating the potential of a cell culture-based model in order to describe the cell-specific invasive properties and to provide a list of putative cancer biomarkers.

Original languageEnglish
Pages (from-to)2577-2587
Number of pages11
JournalJournal of Cellular Biochemistry
Issue number11
Publication statusPublished - Nov 2013


  • CACO-2
  • Colon Cancer
  • Proteomics
  • Secretome

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'The secretome signature of colon cancer cell lines'. Together they form a unique fingerprint.

  • Cite this

    Imperlini, E., Colavita, I., Caterino, M., Mirabelli, P., Pagnozzi, D., Vecchio, L. D., Noto, R. D., Ruoppolo, M., & Orrù, S. (2013). The secretome signature of colon cancer cell lines. Journal of Cellular Biochemistry, 114(11), 2577-2587.