The selected reaction monitoring/multiple reaction monitoring-based mass spectrometry approach for the accurate quantitation of proteins: Clinical applications in the cardiovascular diseases

Research output: Contribution to journalArticlepeer-review

Abstract

Selected reaction monitoring, also known as multiple reaction monitoring, is a powerful targeted mass spectrometry approach for a confident quantitation of proteins/peptides in complex biological samples. In recent years, its optimization and application have become pivotal and of great interest in clinical research to derive useful outcomes for patient care. Thus, selected reaction monitoring/multiple reaction monitoring is now used as a highly sensitive and selective method for the evaluation of protein abundances and biomarker verification with potential applications in medical screening. This review describes technical aspects for the development of a robust multiplex assay and discussing its recent applications in cardiovascular proteomics: verification of promising disease candidates to select only the highest quality peptides/proteins for a preclinical validation, as well as quantitation of protein isoforms and post-translational modifications.

Original languageEnglish
Pages (from-to)771-788
Number of pages18
JournalExpert Review of Proteomics
Volume11
Issue number6
DOIs
Publication statusPublished - Dec 1 2014

Keywords

  • biomarkers
  • cardiovascular diseases
  • endogenous proteins
  • mass spectrometry
  • multiple reaction monitoring
  • post-translational modifications
  • protein isoforms
  • selected reaction monitoring
  • targeted proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Medicine(all)

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