Abstract
The capacity to solubilize immune complexes can be readily measured by incubating the test serum with a suspension of an immune precipitated formed by β-galactosidase and anti-β-galactosidase antibody, and then reading the enzyme units (EU) liberated in the clear supernatant. Our method is rapid and inexpensive; it can be performed in plates and read in scanning colorimeters. Although on large numbers of observations the ICSC is significantly correlated with the CH50, a few discordant cases suggest that solubilization and haemolysis are functions of the alternative and classical pathways of complement respectively.
| Original language | English |
|---|---|
| Pages (from-to) | 119-130 |
| Number of pages | 12 |
| Journal | Journal of Immunological Methods |
| Volume | 77 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 28 1985 |
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Keywords
- complement
- immune complexes
- solubilization
- β-galactosidase
ASJC Scopus subject areas
- Biotechnology
- Immunology
Cite this
The serum capacity to solubilize immune complexes (ICSC) measured by an enzyme-anti-enzyme complex probe. / Migliorini, Paola; Chieregatti, Giorgio; Trovatello, Giorgio; Cantarella, Sebastiana; Fenoglio, Daniela; Celada, Franco.
In: Journal of Immunological Methods, Vol. 77, No. 1, 28.02.1985, p. 119-130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The serum capacity to solubilize immune complexes (ICSC) measured by an enzyme-anti-enzyme complex probe
AU - Migliorini, Paola
AU - Chieregatti, Giorgio
AU - Trovatello, Giorgio
AU - Cantarella, Sebastiana
AU - Fenoglio, Daniela
AU - Celada, Franco
PY - 1985/2/28
Y1 - 1985/2/28
N2 - The capacity to solubilize immune complexes can be readily measured by incubating the test serum with a suspension of an immune precipitated formed by β-galactosidase and anti-β-galactosidase antibody, and then reading the enzyme units (EU) liberated in the clear supernatant. Our method is rapid and inexpensive; it can be performed in plates and read in scanning colorimeters. Although on large numbers of observations the ICSC is significantly correlated with the CH50, a few discordant cases suggest that solubilization and haemolysis are functions of the alternative and classical pathways of complement respectively.
AB - The capacity to solubilize immune complexes can be readily measured by incubating the test serum with a suspension of an immune precipitated formed by β-galactosidase and anti-β-galactosidase antibody, and then reading the enzyme units (EU) liberated in the clear supernatant. Our method is rapid and inexpensive; it can be performed in plates and read in scanning colorimeters. Although on large numbers of observations the ICSC is significantly correlated with the CH50, a few discordant cases suggest that solubilization and haemolysis are functions of the alternative and classical pathways of complement respectively.
KW - complement
KW - immune complexes
KW - solubilization
KW - β-galactosidase
UR - http://www.scopus.com/inward/record.url?scp=0021946005&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021946005&partnerID=8YFLogxK
U2 - 10.1016/0022-1759(85)90189-9
DO - 10.1016/0022-1759(85)90189-9
M3 - Article
C2 - 3919105
AN - SCOPUS:0021946005
VL - 77
SP - 119
EP - 130
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
IS - 1
ER -