The serum capacity to solubilize immune complexes (ICSC) measured by an enzyme-anti-enzyme complex probe

Paola Migliorini, Giorgio Chieregatti, Giorgio Trovatello, Sebastiana Cantarella, Daniela Fenoglio, Franco Celada

Research output: Contribution to journalArticlepeer-review

Abstract

The capacity to solubilize immune complexes can be readily measured by incubating the test serum with a suspension of an immune precipitated formed by β-galactosidase and anti-β-galactosidase antibody, and then reading the enzyme units (EU) liberated in the clear supernatant. Our method is rapid and inexpensive; it can be performed in plates and read in scanning colorimeters. Although on large numbers of observations the ICSC is significantly correlated with the CH50, a few discordant cases suggest that solubilization and haemolysis are functions of the alternative and classical pathways of complement respectively.

Original languageEnglish
Pages (from-to)119-130
Number of pages12
JournalJournal of Immunological Methods
Volume77
Issue number1
DOIs
Publication statusPublished - Feb 28 1985

Keywords

  • complement
  • immune complexes
  • solubilization
  • β-galactosidase

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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