The serum capacity to solubilize immune complexes (ICSC) measured by an enzyme-anti-enzyme complex probe

Paola Migliorini, Giorgio Chieregatti, Giorgio Trovatello, Sebastiana Cantarella, Daniela Fenoglio, Franco Celada

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The capacity to solubilize immune complexes can be readily measured by incubating the test serum with a suspension of an immune precipitated formed by β-galactosidase and anti-β-galactosidase antibody, and then reading the enzyme units (EU) liberated in the clear supernatant. Our method is rapid and inexpensive; it can be performed in plates and read in scanning colorimeters. Although on large numbers of observations the ICSC is significantly correlated with the CH50, a few discordant cases suggest that solubilization and haemolysis are functions of the alternative and classical pathways of complement respectively.

Original languageEnglish
Pages (from-to)119-130
Number of pages12
JournalJournal of Immunological Methods
Volume77
Issue number1
DOIs
Publication statusPublished - Feb 28 1985

Fingerprint

Galactosidases
Antigen-Antibody Complex
Classical Complement Pathway
Alternative Complement Pathway
Enzymes
Hemolysis
Serum
Reading
Anti-Idiotypic Antibodies
Suspensions

Keywords

  • complement
  • immune complexes
  • solubilization
  • β-galactosidase

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

The serum capacity to solubilize immune complexes (ICSC) measured by an enzyme-anti-enzyme complex probe. / Migliorini, Paola; Chieregatti, Giorgio; Trovatello, Giorgio; Cantarella, Sebastiana; Fenoglio, Daniela; Celada, Franco.

In: Journal of Immunological Methods, Vol. 77, No. 1, 28.02.1985, p. 119-130.

Research output: Contribution to journalArticle

Migliorini, Paola ; Chieregatti, Giorgio ; Trovatello, Giorgio ; Cantarella, Sebastiana ; Fenoglio, Daniela ; Celada, Franco. / The serum capacity to solubilize immune complexes (ICSC) measured by an enzyme-anti-enzyme complex probe. In: Journal of Immunological Methods. 1985 ; Vol. 77, No. 1. pp. 119-130.
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