The shc family protein adaptor, Rai, negatively regulates T cell antigen receptor signaling by inhibiting ZAP-70 recruitment and activation

Micol Ferro, Maria Teresa Savino, Barbara Ortensi, Francesca Finetti, Luca Genovese, Giulia Masi, Cristina Ulivieri, Daniela Benati, Giuliana Pelicci, Cosima T. Baldari

Research output: Contribution to journalArticle

Abstract

Rai/ShcC is a member of the Shc family of protein adaptors expressed with the highest abundance in the central nervous system, where it exerts a protective function by coupling neurotrophic receptors to the PI3K/Akt survival pathway. Rai is also expressed, albeit at lower levels, in other cell types, including T and B lymphocytes. We have previously reported that in these cells Rai attenuates antigen receptor signaling, thereby impairing not only cell proliferation but also, opposite to neurons, cell survival. Here we have addressed the mechanism underlying the inhibitory activity of Rai on TCR signaling. We show that Rai interferes with the TCR signaling cascade one of the earliest steps -recruitment of the initiating kinase ZAP-70 to the phosphorylated subunit of the TCR/CD3 complex, which results in a generalized dampening of the downstream signaling events. The inhibitory activity of Rai is associated to its inducible recruitment to phosphorylated CD3, which occurs in the physiological signaling context of the immune synapse. Rai is moreover found as a pre-assembled complex with ZAP-70 and also constitutively interacts with the regulatory p85 subunit of PI3K, similar to neuronal cells, notwithstanding the opposite biological outcome, i.e. impairment of PI-3K/Akt activation. The data highlight the ability of Rai to establish interactions with the TCR and key signaling mediators which, either directly (e.g. by inhibiting ZAP-70 recruitment to the TCR or sequestering ZAP-70/PI3K in the cytosol) or indirectly (e.g. by promoting the recruitment of effectors responsible for signal extinction) prevent full triggering of the TCR signaling cascade.

Original languageEnglish
Article numbere29899
JournalPLoS One
Volume6
Issue number12
DOIs
Publication statusPublished - Dec 29 2011

Fingerprint

Shc Signaling Adaptor Proteins
phosphatidylinositol 3-kinase
T-Cell Antigen Receptor
Phosphatidylinositol 3-Kinases
T-lymphocytes
Chemical activation
antigens
receptors
Proteins
ZAP-70 Protein-Tyrosine Kinase
proteins
neurons
T-Cell Antigen Receptor-CD3 Complex
Antigen Receptors
Aptitude
synapse
cytosol
Cytosol
Synapses
B-lymphocytes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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The shc family protein adaptor, Rai, negatively regulates T cell antigen receptor signaling by inhibiting ZAP-70 recruitment and activation. / Ferro, Micol; Savino, Maria Teresa; Ortensi, Barbara; Finetti, Francesca; Genovese, Luca; Masi, Giulia; Ulivieri, Cristina; Benati, Daniela; Pelicci, Giuliana; Baldari, Cosima T.

In: PLoS One, Vol. 6, No. 12, e29899, 29.12.2011.

Research output: Contribution to journalArticle

Ferro, Micol ; Savino, Maria Teresa ; Ortensi, Barbara ; Finetti, Francesca ; Genovese, Luca ; Masi, Giulia ; Ulivieri, Cristina ; Benati, Daniela ; Pelicci, Giuliana ; Baldari, Cosima T. / The shc family protein adaptor, Rai, negatively regulates T cell antigen receptor signaling by inhibiting ZAP-70 recruitment and activation. In: PLoS One. 2011 ; Vol. 6, No. 12.
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AU - Genovese, Luca

AU - Masi, Giulia

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