Abstract
Kcv (K+ Chlorella virus) is a miniature virus-encoded K+ channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.
Original language | English |
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Pages (from-to) | 65-69 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 530 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - Oct 23 2002 |
Keywords
- Chlorella virus PBCV-1
- HEK 293 cell
- K channel
- Kcv
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology