The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv

Anna Moroni, Carlo Viscomi, Vanessa Sangiorgio, Cinzia Pagliuca, Tobias Meckel, Ferenc Horvath, Sabrina Gazzarrini, Paola Valbuzzi, James L. Van Etten, Dario DiFrancesco, Gerhard Thiel

Research output: Contribution to journalArticlepeer-review

Abstract

Kcv (K+ Chlorella virus) is a miniature virus-encoded K+ channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.

Original languageEnglish
Pages (from-to)65-69
Number of pages5
JournalFEBS Letters
Volume530
Issue number1-3
DOIs
Publication statusPublished - Oct 23 2002

Keywords

  • Chlorella virus PBCV-1
  • HEK 293 cell
  • K channel
  • Kcv

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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