Abstract
Kcv (K+ Chlorella virus) is a miniature virus-encoded K+ channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.
| Original language | English |
|---|---|
| Pages (from-to) | 65-69 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 530 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - Oct 23 2002 |
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Keywords
- Chlorella virus PBCV-1
- HEK 293 cell
- K channel
- Kcv
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv. / Moroni, Anna; Viscomi, Carlo; Sangiorgio, Vanessa; Pagliuca, Cinzia; Meckel, Tobias; Horvath, Ferenc; Gazzarrini, Sabrina; Valbuzzi, Paola; Van Etten, James L.; DiFrancesco, Dario; Thiel, Gerhard.
In: FEBS Letters, Vol. 530, No. 1-3, 23.10.2002, p. 65-69.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv
AU - Moroni, Anna
AU - Viscomi, Carlo
AU - Sangiorgio, Vanessa
AU - Pagliuca, Cinzia
AU - Meckel, Tobias
AU - Horvath, Ferenc
AU - Gazzarrini, Sabrina
AU - Valbuzzi, Paola
AU - Van Etten, James L.
AU - DiFrancesco, Dario
AU - Thiel, Gerhard
PY - 2002/10/23
Y1 - 2002/10/23
N2 - Kcv (K+ Chlorella virus) is a miniature virus-encoded K+ channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.
AB - Kcv (K+ Chlorella virus) is a miniature virus-encoded K+ channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.
KW - Chlorella virus PBCV-1
KW - HEK 293 cell
KW - K channel
KW - Kcv
UR - http://www.scopus.com/inward/record.url?scp=18644379916&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=18644379916&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(02)03397-5
DO - 10.1016/S0014-5793(02)03397-5
M3 - Article
C2 - 12387867
AN - SCOPUS:18644379916
VL - 530
SP - 65
EP - 69
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-3
ER -