The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv

Anna Moroni; Carlo Viscomi; Vanessa Sangiorgio; Cinzia Pagliuca; Tobias Meckel; Ferenc Horvath; Sabrina Gazzarrini; Paola Valbuzzi; James L. Van Etten; Dario DiFrancesco; Gerhard Thiel

doi:10.1016/S0014-5793(02)03397-5

The short N-terminus is required for functional expression of the virus-encoded miniature K⁺ channel Kcv

Anna Moroni, Carlo Viscomi, Vanessa Sangiorgio, Cinzia Pagliuca, Tobias Meckel, Ferenc Horvath, Sabrina Gazzarrini, Paola Valbuzzi, James L. Van Etten, Dario DiFrancesco, Gerhard Thiel

Research output: Contribution to journal › Article › peer-review

Abstract

Kcv (K⁺ Chlorella virus) is a miniature virus-encoded K⁺ channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.

Original language	English
Pages (from-to)	65-69
Number of pages	5
Journal	FEBS Letters
Volume	530
Issue number	1-3
DOIs	https://doi.org/10.1016/S0014-5793(02)03397-5
Publication status	Published - Oct 23 2002

Keywords

Chlorella virus PBCV-1
HEK 293 cell
K channel
Kcv

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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The short N-terminus is required for functional expression of the virus-encoded miniature K⁺ channel Kcv. / Moroni, Anna; Viscomi, Carlo; Sangiorgio, Vanessa; Pagliuca, Cinzia; Meckel, Tobias; Horvath, Ferenc; Gazzarrini, Sabrina; Valbuzzi, Paola; Van Etten, James L.; DiFrancesco, Dario; Thiel, Gerhard.

In: FEBS Letters, Vol. 530, No. 1-3, 23.10.2002, p. 65-69.

Research output: Contribution to journal › Article › peer-review

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abstract = "Kcv (K+ Chlorella virus) is a miniature virus-encoded K+ channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.",

keywords = "Chlorella virus PBCV-1, HEK 293 cell, K channel, Kcv",

author = "Anna Moroni and Carlo Viscomi and Vanessa Sangiorgio and Cinzia Pagliuca and Tobias Meckel and Ferenc Horvath and Sabrina Gazzarrini and Paola Valbuzzi and {Van Etten}, {James L.} and Dario DiFrancesco and Gerhard Thiel",

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T1 - The short N-terminus is required for functional expression of the virus-encoded miniature K+ channel Kcv

AU - Moroni, Anna

AU - Viscomi, Carlo

AU - Sangiorgio, Vanessa

AU - Pagliuca, Cinzia

AU - Meckel, Tobias

AU - Horvath, Ferenc

AU - Gazzarrini, Sabrina

AU - Valbuzzi, Paola

AU - Van Etten, James L.

AU - DiFrancesco, Dario

AU - Thiel, Gerhard

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AB - Kcv (K+ Chlorella virus) is a miniature virus-encoded K+ channel. Its predicted membrane-pore-membrane structure lacks a cytoplasmic C-terminus and it has a short 12 amino acid (aa) cytoplasmic N-terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N-terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N-terminus controls functional properties of the Kcv channel, but does not influence protein expression.

KW - Chlorella virus PBCV-1

KW - HEK 293 cell

KW - K channel

KW - Kcv

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