The Signaling Adaptor Eps8 Is an Essential Actin Capping Protein for Dendritic Cell Migration

Emanuela Frittoli, Gianluca Matteoli, Andrea Palamidessi, Elisa Mazzini, Luigi Maddaluno, Andrea Disanza, Changsong Yang, Tatyana Svitkina, Maria Rescigno, Giorgio Scita

Research output: Contribution to journalArticle

Abstract

Dendritic cells (DCs) flexibly adapt to different microenvironments by using diverse migration strategies that are ultimately dependent on the dynamics and structural organization of the actin cytoskeleton. Here, we have shown that DCs require the actin capping activity of the signaling adaptor Eps8 to polarize and to form elongated migratory protrusions. DCs from Eps8-deficient mice are impaired in directional and chemotactic migration in 3D in vitro and are delayed in reaching the draining lymph node (DLN) in vivo after inflammatory challenge. Hence, Eps8-deficient mice are unable to mount a contact hypersensitivity response. We have also shown that the DC migratory defect is cell autonomous and that Eps8 is required for the proper architectural organization of the actin meshwork and dynamics of cell protrusions. Yet, Eps8 is not necessary for antigen uptake, processing, and presentation. Thus, we have identified Eps8 as a unique actin capping protein specifically required for DC migration.

Original languageEnglish
Pages (from-to)388-399
Number of pages12
JournalImmunity
Volume35
Issue number3
DOIs
Publication statusPublished - Sep 23 2011

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Actin Capping Proteins
Dendritic Cells
Cell Movement
Antigen Presentation
Actins
Contact Dermatitis
Actin Cytoskeleton
Lymph Nodes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Frittoli, E., Matteoli, G., Palamidessi, A., Mazzini, E., Maddaluno, L., Disanza, A., ... Scita, G. (2011). The Signaling Adaptor Eps8 Is an Essential Actin Capping Protein for Dendritic Cell Migration. Immunity, 35(3), 388-399. https://doi.org/10.1016/j.immuni.2011.07.007

The Signaling Adaptor Eps8 Is an Essential Actin Capping Protein for Dendritic Cell Migration. / Frittoli, Emanuela; Matteoli, Gianluca; Palamidessi, Andrea; Mazzini, Elisa; Maddaluno, Luigi; Disanza, Andrea; Yang, Changsong; Svitkina, Tatyana; Rescigno, Maria; Scita, Giorgio.

In: Immunity, Vol. 35, No. 3, 23.09.2011, p. 388-399.

Research output: Contribution to journalArticle

Frittoli, E, Matteoli, G, Palamidessi, A, Mazzini, E, Maddaluno, L, Disanza, A, Yang, C, Svitkina, T, Rescigno, M & Scita, G 2011, 'The Signaling Adaptor Eps8 Is an Essential Actin Capping Protein for Dendritic Cell Migration', Immunity, vol. 35, no. 3, pp. 388-399. https://doi.org/10.1016/j.immuni.2011.07.007
Frittoli E, Matteoli G, Palamidessi A, Mazzini E, Maddaluno L, Disanza A et al. The Signaling Adaptor Eps8 Is an Essential Actin Capping Protein for Dendritic Cell Migration. Immunity. 2011 Sep 23;35(3):388-399. https://doi.org/10.1016/j.immuni.2011.07.007
Frittoli, Emanuela ; Matteoli, Gianluca ; Palamidessi, Andrea ; Mazzini, Elisa ; Maddaluno, Luigi ; Disanza, Andrea ; Yang, Changsong ; Svitkina, Tatyana ; Rescigno, Maria ; Scita, Giorgio. / The Signaling Adaptor Eps8 Is an Essential Actin Capping Protein for Dendritic Cell Migration. In: Immunity. 2011 ; Vol. 35, No. 3. pp. 388-399.
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