TY - JOUR
T1 - The small chemical vacuolin-1 inhibits Ca2+-dependent lysosomal exocytosis but not cell resealing
AU - Cerny, Jan
AU - Feng, Yan
AU - Yu, Anan
AU - Miyake, Katsuya
AU - Borgonovo, Barbara
AU - Klumperman, Judith
AU - Meldolesi, Jacopo
AU - McNeil, Paul L.
AU - Kirchhausen, Tomas
PY - 2004/9
Y1 - 2004/9
N2 - Resealing after wounding, the process of repair following plasma membrane damage, requires exocytosis. Vacuolins are molecules that induce rapid formation of large, swollen structures derived from endosomes and lysosomes by homotypic fusion combined with uncontrolled fusion of the inner and limiting membranes of these organelles. Vacuolin-1, the most potent compound, blocks the Ca2+- dependent exocytosis of lysosomes induced by ionomycin or plasma membrane wounding, without affecting the process of resealing. In contrast, other cell structures and membrane trafficking functions including exocytosis of enlargeosomes are unaffected. Because cells heal normally in the presence of vacuolin-1, we suggest that lysosomes are dispensable for resealing.
AB - Resealing after wounding, the process of repair following plasma membrane damage, requires exocytosis. Vacuolins are molecules that induce rapid formation of large, swollen structures derived from endosomes and lysosomes by homotypic fusion combined with uncontrolled fusion of the inner and limiting membranes of these organelles. Vacuolin-1, the most potent compound, blocks the Ca2+- dependent exocytosis of lysosomes induced by ionomycin or plasma membrane wounding, without affecting the process of resealing. In contrast, other cell structures and membrane trafficking functions including exocytosis of enlargeosomes are unaffected. Because cells heal normally in the presence of vacuolin-1, we suggest that lysosomes are dispensable for resealing.
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U2 - 10.1038/sj.embor.7400243
DO - 10.1038/sj.embor.7400243
M3 - Article
C2 - 15332114
AN - SCOPUS:9444225430
VL - 5
SP - 883
EP - 888
JO - EMBO Reports
JF - EMBO Reports
SN - 1469-221X
IS - 9
ER -