The specific inhibitor of protein kinase C, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), induces morphological change and cell differentiation of human neural crest-derived cell lineages

Maria Teresa Parodi; Luigi Varesio; Gian Paolo Tonini

doi:10.1016/0014-5793(90)81104-V

The specific inhibitor of protein kinase C, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), induces morphological change and cell differentiation of human neural crest-derived cell lineages

Maria Teresa Parodi, Luigi Varesio, Gian Paolo Tonini

Istituto "Giannina Gaslini"

Research output: Contribution to journal › Article › peer-review

Abstract

It has been proved that inhibition of protein kinase C by 1-(5-isoquinolinylsulfonyl)-1-methylpiperazine (H7) induces morphological differentiation in murine neuroblastoma (nb) cell. Here we report that H7 is also active on human nb cell lines. The human nb cell had originally neuroblast-like (N) or intermediate (I) morphology. N and I type are thought to represent different stages of neuroblastoma differentiation. Neurite outgrowth was observed in both N and I type morphology treating the cells with 7, 14 or 28 μM of H7. The results confirm previous observations and show that inhibition of PKC by H7 also promotes neuronal differentiation in human cell line variants.

Original language	English
Pages (from-to)	4-6
Number of pages	3
Journal	FEBS Letters
Volume	269
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(90)81104-V
Publication status	Published - Aug 20 1990

Keywords

1-(5-Isoquinolinylsulfonyl)-2-methylpiperazine (H7)
Human neuroblastoma
Neural differentiation
Protein kinase C

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/0014-5793(90)81104-V

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The specific inhibitor of protein kinase C, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), induces morphological change and cell differentiation of human neural crest-derived cell lineages. / Parodi, Maria Teresa; Varesio, Luigi; Tonini, Gian Paolo.

In: FEBS Letters, Vol. 269, No. 1, 20.08.1990, p. 4-6.

Research output: Contribution to journal › Article › peer-review

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abstract = "It has been proved that inhibition of protein kinase C by 1-(5-isoquinolinylsulfonyl)-1-methylpiperazine (H7) induces morphological differentiation in murine neuroblastoma (nb) cell. Here we report that H7 is also active on human nb cell lines. The human nb cell had originally neuroblast-like (N) or intermediate (I) morphology. N and I type are thought to represent different stages of neuroblastoma differentiation. Neurite outgrowth was observed in both N and I type morphology treating the cells with 7, 14 or 28 μM of H7. The results confirm previous observations and show that inhibition of PKC by H7 also promotes neuronal differentiation in human cell line variants.",

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AU - Tonini, Gian Paolo

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N2 - It has been proved that inhibition of protein kinase C by 1-(5-isoquinolinylsulfonyl)-1-methylpiperazine (H7) induces morphological differentiation in murine neuroblastoma (nb) cell. Here we report that H7 is also active on human nb cell lines. The human nb cell had originally neuroblast-like (N) or intermediate (I) morphology. N and I type are thought to represent different stages of neuroblastoma differentiation. Neurite outgrowth was observed in both N and I type morphology treating the cells with 7, 14 or 28 μM of H7. The results confirm previous observations and show that inhibition of PKC by H7 also promotes neuronal differentiation in human cell line variants.

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The specific inhibitor of protein kinase C, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), induces morphological change and cell differentiation of human neural crest-derived cell lineages

Abstract

Keywords

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