Abstract
It has been proved that inhibition of protein kinase C by 1-(5-isoquinolinylsulfonyl)-1-methylpiperazine (H7) induces morphological differentiation in murine neuroblastoma (nb) cell. Here we report that H7 is also active on human nb cell lines. The human nb cell had originally neuroblast-like (N) or intermediate (I) morphology. N and I type are thought to represent different stages of neuroblastoma differentiation. Neurite outgrowth was observed in both N and I type morphology treating the cells with 7, 14 or 28 μM of H7. The results confirm previous observations and show that inhibition of PKC by H7 also promotes neuronal differentiation in human cell line variants.
| Original language | English |
|---|---|
| Pages (from-to) | 4-6 |
| Number of pages | 3 |
| Journal | FEBS Letters |
| Volume | 269 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Aug 20 1990 |
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Keywords
- 1-(5-Isoquinolinylsulfonyl)-2-methylpiperazine (H7)
- Human neuroblastoma
- Neural differentiation
- Protein kinase C
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
The specific inhibitor of protein kinase C, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), induces morphological change and cell differentiation of human neural crest-derived cell lineages. / Parodi, Maria Teresa; Varesio, Luigi; Tonini, Gian Paolo.
In: FEBS Letters, Vol. 269, No. 1, 20.08.1990, p. 4-6.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The specific inhibitor of protein kinase C, 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), induces morphological change and cell differentiation of human neural crest-derived cell lineages
AU - Parodi, Maria Teresa
AU - Varesio, Luigi
AU - Tonini, Gian Paolo
PY - 1990/8/20
Y1 - 1990/8/20
N2 - It has been proved that inhibition of protein kinase C by 1-(5-isoquinolinylsulfonyl)-1-methylpiperazine (H7) induces morphological differentiation in murine neuroblastoma (nb) cell. Here we report that H7 is also active on human nb cell lines. The human nb cell had originally neuroblast-like (N) or intermediate (I) morphology. N and I type are thought to represent different stages of neuroblastoma differentiation. Neurite outgrowth was observed in both N and I type morphology treating the cells with 7, 14 or 28 μM of H7. The results confirm previous observations and show that inhibition of PKC by H7 also promotes neuronal differentiation in human cell line variants.
AB - It has been proved that inhibition of protein kinase C by 1-(5-isoquinolinylsulfonyl)-1-methylpiperazine (H7) induces morphological differentiation in murine neuroblastoma (nb) cell. Here we report that H7 is also active on human nb cell lines. The human nb cell had originally neuroblast-like (N) or intermediate (I) morphology. N and I type are thought to represent different stages of neuroblastoma differentiation. Neurite outgrowth was observed in both N and I type morphology treating the cells with 7, 14 or 28 μM of H7. The results confirm previous observations and show that inhibition of PKC by H7 also promotes neuronal differentiation in human cell line variants.
KW - 1-(5-Isoquinolinylsulfonyl)-2-methylpiperazine (H7)
KW - Human neuroblastoma
KW - Neural differentiation
KW - Protein kinase C
UR - http://www.scopus.com/inward/record.url?scp=0024995709&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024995709&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(90)81104-V
DO - 10.1016/0014-5793(90)81104-V
M3 - Article
C2 - 2387413
AN - SCOPUS:0024995709
VL - 269
SP - 4
EP - 6
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -