The staphylococcal respiratory response regulator SrrAB induces ica gene transcription and polysaccharide intercellular adhesin expression, protecting Staphylococcus aureus from neutrophil killing under anaerobic growth conditions

Martina Ulrich, Mike Bastian, Sarah E. Cramton, Katrin Ziegler, Alexa A. Pragman, Alessandra Bragonzi, Guido Memmi, Christiane Wolz, Patrick M. Schlievert, Ambrose Cheung, Gerd Döring

Research output: Contribution to journalArticle

Abstract

In anaerobic environments, Staphylococcus aureus increases the transcription of the intercellular adhesin (ica) cluster, leading to increased polysaccharide intercellular adhesin (PIA) production. The regulatory mechanisms involved in this phenotypic change are mostly unknown. Here we show that the staphylococcal respiratory response regulator, SrrAB, significantly increases icaA transcription under anaerobic growth in S. aureus. Phosphorylated SrrA preferentially bound to a 100 bp DNA sequence located upstream of ica, and dot blot assays revealed little or no PIA expression in S. aureus srrAB deletion-replacement mutants of strains Sa113 and SH1000, grown anaerobically. The biological relevance of SrrAB for S. aureus was assessed in a phagocytosis assay employing human neutrophils. Sixty-eight per cent of PIA producing wild-type cells, but only 19% of srrAB mutant cells survived under anaerobic conditions, suggesting that PIA protected S. aureus against non-oxidative killing mechanisms of the neutrophils. No protection was observed when S. aureus or S. epidermidis strains, producing PIA also under aerobic conditions, were subjected to phagocytosis under aerobic conditions. These results demonstrate that SrrAB is a major activator of ica expression and PIA production in anaerobic environments, where it contributes to the protection of S. aureus against non-oxidative defence mechanisms.

Original languageEnglish
Pages (from-to)1276-1287
Number of pages12
JournalMolecular Microbiology
Volume65
Issue number5
DOIs
Publication statusPublished - Sep 2007

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

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