The Ste locus, a component of the parasitic cry-Ste system of Drosophila melanogaster, encodes a protein that forms crystals in primary spermatocytes and mimics properties of the β subunit of casein kinase 2

Maria P. Bozzetti, Sara Massari, Palma Finelli, Flavio Meggio, Lorenzo A. Pinna, Brigitte Boldyreff, Olaf G. Issinger, Gioacchino Palumbo, Clara Ciriaco, Silvia Bonaccorsi, Sergio Pimpinelli

Research output: Contribution to journalArticlepeer-review

Abstract

Males of Drosophila melanogaster lacking the Y chromosome-linked crystal locus show multiple meiotic alterations, including chromosome disorganization and prominent crystal formation in primary spermatocytes. These alterations are due to the derepression of the X chromosome-linked Stellate sequences. To understand how the derepression of the Stellate elements gives rise to these abnormalities, we have expressed the protein encoded by the Stellate sequences in bacteria and produced an antibody against the fusion protein. Immunostaining of crystal- testes has clearly shown that the Stellate protein is a major component of the crystals. Moreover, in vitro experiments have shown that this protein can interact with the catalytic α subunit of casein kinase 2 enzyme, altering its activity.

Original languageEnglish
Pages (from-to)6067-6071
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number13
Publication statusPublished - Jun 20 1995

Keywords

  • Meiosis
  • Repetitive DNA

ASJC Scopus subject areas

  • General
  • Genetics

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