Abstract
Lipids are an important energy supply in our cells and can be stored or used to produce macromolecules during lipogenesis when cells experience nutrient starvation. Our proteomic analysis reveals that the Drosophila homologue of human Stearoyl-CoA desaturase-1 (Desat1) is an indirect target of Myc in fat cells. Stearoyl-CoA desaturases are key enzymes in the synthesis of monounsaturated fatty acids critical for the formation of complex lipids such as triglycerides and phospholipids. Their function is fundamental for cellular physiology, however in tumors, overexpression of SCD-1 and SCD-5 has been found frequently associated with a poor prognosis. Another gene that is often upregulated in tumors is the proto-oncogene c-myc, where its overexpression or increased protein stability, favor cellular growth. Here, we report a potential link between Myc and Desat1 to control autophagy and growth. Using Drosophila, we found that expression of Desat1, in metabolic tissues like the fat body, in the gut and in epithelial cells, is necessary for Myc function to induce autophagy a cell eating mechanism important for energy production. In addition, we observed that reduction of Desat1 affects Myc ability to induce growth in epithelial cells. Our data also identify, in pancreatic tumor cells, a significant correlation between the expression of Myc and SCD-1 proteins, suggesting the existence of a potential functional relationship between the activities of these proteins in sustaining tumor progression.
| Original language | English |
|---|---|
| Article number | 131 |
| Journal | Genes |
| Volume | 8 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - May 1 2017 |
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Keywords
- Autophagy
- Drosophila
- Growth
- Lipid metabolism
- Myc
- Prostate tumors
- SCD-1/Desat1
ASJC Scopus subject areas
- Genetics
- Genetics(clinical)
Cite this
The stearoyl-CoA desaturase-1 (Desat1) in Drosophila cooperated with Myc to induce autophagy and growth, a potential new link to tumor survival. / Paiardi, Chiara; Mirzoyan, Zhasmine; Zola, Sheri; Parisi, Federica; Vingiani, Andrea; Pasini, Maria Enrica; Bellosta, Paola.
In: Genes, Vol. 8, No. 5, 131, 01.05.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The stearoyl-CoA desaturase-1 (Desat1) in Drosophila cooperated with Myc to induce autophagy and growth, a potential new link to tumor survival
AU - Paiardi, Chiara
AU - Mirzoyan, Zhasmine
AU - Zola, Sheri
AU - Parisi, Federica
AU - Vingiani, Andrea
AU - Pasini, Maria Enrica
AU - Bellosta, Paola
PY - 2017/5/1
Y1 - 2017/5/1
N2 - Lipids are an important energy supply in our cells and can be stored or used to produce macromolecules during lipogenesis when cells experience nutrient starvation. Our proteomic analysis reveals that the Drosophila homologue of human Stearoyl-CoA desaturase-1 (Desat1) is an indirect target of Myc in fat cells. Stearoyl-CoA desaturases are key enzymes in the synthesis of monounsaturated fatty acids critical for the formation of complex lipids such as triglycerides and phospholipids. Their function is fundamental for cellular physiology, however in tumors, overexpression of SCD-1 and SCD-5 has been found frequently associated with a poor prognosis. Another gene that is often upregulated in tumors is the proto-oncogene c-myc, where its overexpression or increased protein stability, favor cellular growth. Here, we report a potential link between Myc and Desat1 to control autophagy and growth. Using Drosophila, we found that expression of Desat1, in metabolic tissues like the fat body, in the gut and in epithelial cells, is necessary for Myc function to induce autophagy a cell eating mechanism important for energy production. In addition, we observed that reduction of Desat1 affects Myc ability to induce growth in epithelial cells. Our data also identify, in pancreatic tumor cells, a significant correlation between the expression of Myc and SCD-1 proteins, suggesting the existence of a potential functional relationship between the activities of these proteins in sustaining tumor progression.
AB - Lipids are an important energy supply in our cells and can be stored or used to produce macromolecules during lipogenesis when cells experience nutrient starvation. Our proteomic analysis reveals that the Drosophila homologue of human Stearoyl-CoA desaturase-1 (Desat1) is an indirect target of Myc in fat cells. Stearoyl-CoA desaturases are key enzymes in the synthesis of monounsaturated fatty acids critical for the formation of complex lipids such as triglycerides and phospholipids. Their function is fundamental for cellular physiology, however in tumors, overexpression of SCD-1 and SCD-5 has been found frequently associated with a poor prognosis. Another gene that is often upregulated in tumors is the proto-oncogene c-myc, where its overexpression or increased protein stability, favor cellular growth. Here, we report a potential link between Myc and Desat1 to control autophagy and growth. Using Drosophila, we found that expression of Desat1, in metabolic tissues like the fat body, in the gut and in epithelial cells, is necessary for Myc function to induce autophagy a cell eating mechanism important for energy production. In addition, we observed that reduction of Desat1 affects Myc ability to induce growth in epithelial cells. Our data also identify, in pancreatic tumor cells, a significant correlation between the expression of Myc and SCD-1 proteins, suggesting the existence of a potential functional relationship between the activities of these proteins in sustaining tumor progression.
KW - Autophagy
KW - Drosophila
KW - Growth
KW - Lipid metabolism
KW - Myc
KW - Prostate tumors
KW - SCD-1/Desat1
UR - http://www.scopus.com/inward/record.url?scp=85018379891&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85018379891&partnerID=8YFLogxK
U2 - 10.3390/genes8050131
DO - 10.3390/genes8050131
M3 - Article
VL - 8
JO - Genes
JF - Genes
SN - 2073-4425
IS - 5
M1 - 131
ER -