The stimulation of arginine transport by TNFα in human endothelial cells depends on NF-κB activation

Rossana Visigalli, Ovidio Bussolati, Roberto Sala, Amelia Barilli, Bianca Maria Rotoli, Alessandro Parolari, Francesco Alamanni, Gian C. Gazzola, Valeria Dall'Asta

Research output: Contribution to journalArticle

Abstract

In human saphenous vein endothelial cells (HSVECs), tumor necrosis factor-α (TNFα) and bacterial lipopolysaccharide (LPS), but neither interferon γ (IFNγ) nor interleukin 1β (IL-1β), stimulate arginine transport. The effects of TNFα and LPS are due solely to the enhancement of system y+ activity, whereas system y+L is substantially unaffected. TNFα causes an increased expression of SLC7A2/CAT-2B gene while SLC7A1/CAT-1 expression is not altered by the cytokine. The suppression of PKC-dependent transduction pathways, obtained with the inhibitor chelerytrhine, the inhibitor peptide of PKCζ isoform, or chronic exposure to phorbol esters, does not prevent TNFα effect on arginine transport. Likewise, ERK, JNK, and p38 MAP kinases are not involved in the cytokine effect, since arginine transport stimulation is unaffected by their specific inhibitors. On the contrary, inhibitors of NF-κB pathway hinder the increase in CAT2B mRNA and the stimulation of arginine uptake. These results indicate that in human endothelial cells the activation of NF-κB pathway mediates the TNFα effects on arginine transport.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalBBA - Biomembranes
Volume1664
Issue number1
DOIs
Publication statusPublished - Jul 1 2004

Keywords

  • 2-(4-chlorophenyl)-4-(4-fluorophenyl)-5-pyridin-4-yl-1, 2-dihydropyrazol-3-one
  • 5,6-diclorobenzimidazole riboside
  • caffeic acid phenethyl ester
  • CAPE
  • CAT transporter
  • CFPD
  • DRB
  • Earle's balanced salt solution
  • EBSS
  • FBS
  • NFκB
  • SLC7 gene
  • TNFα

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

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  • Cite this

    Visigalli, R., Bussolati, O., Sala, R., Barilli, A., Rotoli, B. M., Parolari, A., Alamanni, F., Gazzola, G. C., & Dall'Asta, V. (2004). The stimulation of arginine transport by TNFα in human endothelial cells depends on NF-κB activation. BBA - Biomembranes, 1664(1), 45-52. https://doi.org/10.1016/j.bbamem.2004.04.001