The striatal-enriched protein Rhes is a critical modulator of cocaine-induced molecular and behavioral responses

Francesco Napolitano, Arianna De Rosa, Rosita Russo, Anna Di Maio, Martina Garofalo, Mauro Federici, Sara Migliarini, Ada Ledonne, Francesca Romana Rizzo, Luigi Avallone, Tommaso Nuzzo, Tommaso Biagini, Massimo Pasqualetti, Nicola Biagio Mercuri, Tommaso Mazza, Angela Chambery, Alessandro Usiello

Research output: Contribution to journalArticle

Abstract

Previous evidence pointed out a role for the striatal-enriched protein Rhes in modulating dopaminergic transmission. Based on the knowledge that cocaine induces both addiction and motor stimulation, through its ability to enhance dopaminergic signaling in the corpus striatum, we have now explored the involvement of Rhes in the effects associated with this psychostimulant. Our behavioral data showed that a lack of Rhes in knockout animals caused profound alterations in motor stimulation following cocaine exposure, eliciting a significant leftward shift in the dose-response curve and triggering a dramatic hyperactivity. We also found that Rhes modulated either short- or long-term motor sensitization induced by cocaine, since lack of this protein prevents both of them in mutants. Consistent with this in vivo observation, we found that lack of Rhes in mice caused a greater increase in striatal cocaine-dependent D1R/cAMP/PKA signaling, along with considerable enhancement of Arc, zif268, and Homer1 mRNA expression. We also documented that lack of Rhes in mice produced cocaine-related striatal alterations in proteomic profiling, with a differential expression of proteins clustering in calcium homeostasis and cytoskeletal protein binding categories. Despite dramatic striatal alterations associated to cocaine exposure, our data did not reveal any significant changes in midbrain dopaminergic neurons as a lack of Rhes did not affect: (i) DAT activity; (ii) D2R-dependent regulation of GIRK; and (iii) D2R-dependent regulation of dopamine release. Collectively, our results strengthen the view that Rhes acts as a pivotal physiological “molecular brake” for striatal dopaminergic system overactivation induced by psychostimulants, thus making this protein of interest in regulating the molecular mechanism underpinning cocaine-dependent motor stimulatory effects.

Original languageEnglish
Article number15294
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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Corpus Striatum
Cocaine
Proteins
Cytoskeletal Proteins
Dopaminergic Neurons
Mesencephalon
Protein Binding
Proteomics
Cluster Analysis
Dopamine
Homeostasis
Observation
Calcium
Messenger RNA

ASJC Scopus subject areas

  • General

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The striatal-enriched protein Rhes is a critical modulator of cocaine-induced molecular and behavioral responses. / Napolitano, Francesco; De Rosa, Arianna; Russo, Rosita; Di Maio, Anna; Garofalo, Martina; Federici, Mauro; Migliarini, Sara; Ledonne, Ada; Rizzo, Francesca Romana; Avallone, Luigi; Nuzzo, Tommaso; Biagini, Tommaso; Pasqualetti, Massimo; Mercuri, Nicola Biagio; Mazza, Tommaso; Chambery, Angela; Usiello, Alessandro.

In: Scientific Reports, Vol. 9, No. 1, 15294, 01.12.2019.

Research output: Contribution to journalArticle

Napolitano, F, De Rosa, A, Russo, R, Di Maio, A, Garofalo, M, Federici, M, Migliarini, S, Ledonne, A, Rizzo, FR, Avallone, L, Nuzzo, T, Biagini, T, Pasqualetti, M, Mercuri, NB, Mazza, T, Chambery, A & Usiello, A 2019, 'The striatal-enriched protein Rhes is a critical modulator of cocaine-induced molecular and behavioral responses', Scientific Reports, vol. 9, no. 1, 15294. https://doi.org/10.1038/s41598-019-51839-w
Napolitano, Francesco ; De Rosa, Arianna ; Russo, Rosita ; Di Maio, Anna ; Garofalo, Martina ; Federici, Mauro ; Migliarini, Sara ; Ledonne, Ada ; Rizzo, Francesca Romana ; Avallone, Luigi ; Nuzzo, Tommaso ; Biagini, Tommaso ; Pasqualetti, Massimo ; Mercuri, Nicola Biagio ; Mazza, Tommaso ; Chambery, Angela ; Usiello, Alessandro. / The striatal-enriched protein Rhes is a critical modulator of cocaine-induced molecular and behavioral responses. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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abstract = "Previous evidence pointed out a role for the striatal-enriched protein Rhes in modulating dopaminergic transmission. Based on the knowledge that cocaine induces both addiction and motor stimulation, through its ability to enhance dopaminergic signaling in the corpus striatum, we have now explored the involvement of Rhes in the effects associated with this psychostimulant. Our behavioral data showed that a lack of Rhes in knockout animals caused profound alterations in motor stimulation following cocaine exposure, eliciting a significant leftward shift in the dose-response curve and triggering a dramatic hyperactivity. We also found that Rhes modulated either short- or long-term motor sensitization induced by cocaine, since lack of this protein prevents both of them in mutants. Consistent with this in vivo observation, we found that lack of Rhes in mice caused a greater increase in striatal cocaine-dependent D1R/cAMP/PKA signaling, along with considerable enhancement of Arc, zif268, and Homer1 mRNA expression. We also documented that lack of Rhes in mice produced cocaine-related striatal alterations in proteomic profiling, with a differential expression of proteins clustering in calcium homeostasis and cytoskeletal protein binding categories. Despite dramatic striatal alterations associated to cocaine exposure, our data did not reveal any significant changes in midbrain dopaminergic neurons as a lack of Rhes did not affect: (i) DAT activity; (ii) D2R-dependent regulation of GIRK; and (iii) D2R-dependent regulation of dopamine release. Collectively, our results strengthen the view that Rhes acts as a pivotal physiological “molecular brake” for striatal dopaminergic system overactivation induced by psychostimulants, thus making this protein of interest in regulating the molecular mechanism underpinning cocaine-dependent motor stimulatory effects.",
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AU - De Rosa, Arianna

AU - Russo, Rosita

AU - Di Maio, Anna

AU - Garofalo, Martina

AU - Federici, Mauro

AU - Migliarini, Sara

AU - Ledonne, Ada

AU - Rizzo, Francesca Romana

AU - Avallone, Luigi

AU - Nuzzo, Tommaso

AU - Biagini, Tommaso

AU - Pasqualetti, Massimo

AU - Mercuri, Nicola Biagio

AU - Mazza, Tommaso

AU - Chambery, Angela

AU - Usiello, Alessandro

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N2 - Previous evidence pointed out a role for the striatal-enriched protein Rhes in modulating dopaminergic transmission. Based on the knowledge that cocaine induces both addiction and motor stimulation, through its ability to enhance dopaminergic signaling in the corpus striatum, we have now explored the involvement of Rhes in the effects associated with this psychostimulant. Our behavioral data showed that a lack of Rhes in knockout animals caused profound alterations in motor stimulation following cocaine exposure, eliciting a significant leftward shift in the dose-response curve and triggering a dramatic hyperactivity. We also found that Rhes modulated either short- or long-term motor sensitization induced by cocaine, since lack of this protein prevents both of them in mutants. Consistent with this in vivo observation, we found that lack of Rhes in mice caused a greater increase in striatal cocaine-dependent D1R/cAMP/PKA signaling, along with considerable enhancement of Arc, zif268, and Homer1 mRNA expression. We also documented that lack of Rhes in mice produced cocaine-related striatal alterations in proteomic profiling, with a differential expression of proteins clustering in calcium homeostasis and cytoskeletal protein binding categories. Despite dramatic striatal alterations associated to cocaine exposure, our data did not reveal any significant changes in midbrain dopaminergic neurons as a lack of Rhes did not affect: (i) DAT activity; (ii) D2R-dependent regulation of GIRK; and (iii) D2R-dependent regulation of dopamine release. Collectively, our results strengthen the view that Rhes acts as a pivotal physiological “molecular brake” for striatal dopaminergic system overactivation induced by psychostimulants, thus making this protein of interest in regulating the molecular mechanism underpinning cocaine-dependent motor stimulatory effects.

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