The synergistic activity of thyroid transcription factor 1 and Pax 8 relies on the promoter/enhancer interplay

Stefania Miccadei, Rossana De Leo, Enrico Zammarchi, Pier Giorgio Natali, Donato Civitareale

Research output: Contribution to journalArticlepeer-review

Abstract

The transcription factors, thyroid transcription factor 1 (TTF-1) and Pax 8, play a pivotal role in the transcriptional regulation of the thyroid differentiation marker genes and in the differentiation of the thyroid follicular cells. They have a very restricted tissue distribution, and the thyrocyte is the only cell type with the simultaneous expression of these factors. Here we show that TTF-1 and Pax 8 cooperatively activate their target genes and that their synergistic activity requires the cross-talk between enhancer and gene promoter. We have characterized the cis and trans requirements of the TTF1/Pax 8 synergistic activity on the thyroperoxidase gene. We show that their synergy is also important for thyroglobulin gene transcription.

Original languageEnglish
Pages (from-to)837-846
Number of pages10
JournalMolecular Endocrinology
Volume16
Issue number4
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

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