The synergistic interaction of interferon types I and II leads to marked reduction in severe acute respiratory syndrome-associated coronavirus replication and increase in the expression of mRNAs for interferon-induced proteins

Carolina Scagnolari, Simona Trombetti, Alessia Alberelli, Simona Cicetti, Daniela Bellarosa, Roberta Longo, Alberto Spanò, Elisabetta Riva, Massimo Clementi, Guido Antonelli

Research output: Contribution to journalArticlepeer-review

Abstract

Interferon (IFN)-α, -β and -γ have been shown to be only marginally effective against severe acute respiratory syndrome coronavirus (SARS-CoV) replication in Vero cell lines. We investigated the combination of type I IFNs (IFN-α or -β) and IFN-γ for antiviral activity and found that such combinations synergistically inhibited SARS-CoV replication in Vero cells, using yield reduction assay and the isobologram and combination index methods of Chou and Talalay for evaluation. The highly synergistic anti-SARS-CoV action of type I IFNs and IFN-γ parallels the marked increase in 2′-5′-oligoadenylate synthetase and p56 mRNAs following exposure in Vero cells to either IFN-α or -β and IFN-γ compared with the transcriptional levels obtained after stimulation with either IFN alone. These results demonstrate that SARS-CoV, although only moderately sensitive to the antiviral action of the individual types of IFN, is highly sensitive to a combination of type I and II IFNs, which suggests that such combinations may have potential in the treatment of SARS-CoV infections.

Original languageEnglish
Pages (from-to)156-160
Number of pages5
JournalIntervirology
Volume50
Issue number2
DOIs
Publication statusPublished - Feb 2007

Keywords

  • 2′-5′-Oligoadenylate synthetase
  • Interferon
  • p56
  • Severe acute respiratory syndrome coronavirus

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'The synergistic interaction of interferon types I and II leads to marked reduction in severe acute respiratory syndrome-associated coronavirus replication and increase in the expression of mRNAs for interferon-induced proteins'. Together they form a unique fingerprint.

Cite this