The synthetic purine reversine selectively induces cell death of cancer cells

Marco Piccoli, Giacomo Palazzolo, Erika Conforti, Giuseppe Lamorte, Nadia Papini, Pasquale Creo, Chiara Fania, Raffaella Scaringi, Sonia Bergante, Cristina Tringali, Leda Roncoroni, Stefania Mazzoleni, Luisa Doneda, Rossella Galli, Bruno Venerando, Guido Tettamanti, Cecilia Gelfi, Luigi Anastasia

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The synthetic purine reversine has been shown to possess a dual activity as it promotes the de-differentiation of adult cells, including fibroblasts, into stem-cell-like progenitors, but it also induces cell growth arrest and ultimately cell death of cancer cells, suggesting its possible application as an anti-cancer agent. Aim of this study was to investigate the mechanism underneath reversine selectivity in inducing cell death of cancer cells by a comparative analysis of its effects on several tumor cells and normal dermal fibroblasts. We found that reversine is lethal for all cancer cells studied as it induces cell endoreplication, a process that malignant cells cannot effectively oppose due to aberrations in cell cycle checkpoints. On the other hand, normal cells, like dermal fibroblasts, can control reversine activity by blocking the cell cycle, entering a reversible quiescent state. However, they can be induced to become sensitive to the molecule when key cell cycle proteins, e.g., p53, are silenced.

Original languageEnglish
Pages (from-to)3207-3217
Number of pages11
JournalJournal of Cellular Biochemistry
Volume113
Issue number10
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Cell death
Cell Death
Cells
Fibroblasts
Neoplasms
Cell Cycle Proteins
Endoreduplication
Cell growth
Stem cells
Aberrations
Skin
2-(4-morpholinoanilino)-6-cyclohexylaminopurine
purine
Tumors
Cell Cycle Checkpoints
Cell Differentiation
Cell Cycle
Molecules
Stem Cells
Growth

Keywords

  • cell reprogramming
  • new anti-cancer drugs
  • Reversine
  • small molecules and stem cells

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

The synthetic purine reversine selectively induces cell death of cancer cells. / Piccoli, Marco; Palazzolo, Giacomo; Conforti, Erika; Lamorte, Giuseppe; Papini, Nadia; Creo, Pasquale; Fania, Chiara; Scaringi, Raffaella; Bergante, Sonia; Tringali, Cristina; Roncoroni, Leda; Mazzoleni, Stefania; Doneda, Luisa; Galli, Rossella; Venerando, Bruno; Tettamanti, Guido; Gelfi, Cecilia; Anastasia, Luigi.

In: Journal of Cellular Biochemistry, Vol. 113, No. 10, 10.2012, p. 3207-3217.

Research output: Contribution to journalArticle

Piccoli, M, Palazzolo, G, Conforti, E, Lamorte, G, Papini, N, Creo, P, Fania, C, Scaringi, R, Bergante, S, Tringali, C, Roncoroni, L, Mazzoleni, S, Doneda, L, Galli, R, Venerando, B, Tettamanti, G, Gelfi, C & Anastasia, L 2012, 'The synthetic purine reversine selectively induces cell death of cancer cells', Journal of Cellular Biochemistry, vol. 113, no. 10, pp. 3207-3217. https://doi.org/10.1002/jcb.24197
Piccoli, Marco ; Palazzolo, Giacomo ; Conforti, Erika ; Lamorte, Giuseppe ; Papini, Nadia ; Creo, Pasquale ; Fania, Chiara ; Scaringi, Raffaella ; Bergante, Sonia ; Tringali, Cristina ; Roncoroni, Leda ; Mazzoleni, Stefania ; Doneda, Luisa ; Galli, Rossella ; Venerando, Bruno ; Tettamanti, Guido ; Gelfi, Cecilia ; Anastasia, Luigi. / The synthetic purine reversine selectively induces cell death of cancer cells. In: Journal of Cellular Biochemistry. 2012 ; Vol. 113, No. 10. pp. 3207-3217.
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