The T cell receptor αβ V-J shuffling shows lack of autonomy between the combining site and the constant domain of the receptor chains

Giulia Casorati, André Traunecker, Klaus Karjalainen

Research output: Contribution to journalArticlepeer-review

Abstract

In order to assess the structural independence of the T cell receptor (TCR) combining site from the rest of the molecule we have generated two recombinant chains consisting of a TCR V-J α region linked to the Cβ and a TCR V-J β linked to the Cα. If the V and C domains of the TCR form independent domains, as has been shown for the Ig molecules, we would expect to obtain a functional chimeric TCR. Interestingly, it was found that the shuffled molecules are produced intracellularly in T cell hybridomas, but are not expressed on the cell surface. To explain this failure of the shuffled molecules we propose that the TCR has a more compact structure, compared to the Ig, and that it is indispensable to keep a longitudinal inter-domain contact between the V-J and C portion to have a functional molecule.

Original languageEnglish
Pages (from-to)586-589
Number of pages4
JournalEuropean Journal of Immunology
Volume23
Issue number2
Publication statusPublished - Feb 1993

Keywords

  • Exon shuffling
  • T cell receptor
  • V regions

ASJC Scopus subject areas

  • Immunology

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