The T cell receptor repertoire in psoriatic synovitis is restricted and T lymphocytes expressing the same TCR are present in joint and skin lesions

Lorena Borgato, Antonio Puccetti, Ruggero Beri, Orazio Codella, Antonio Frigo, Sara Simeoni, Maria Luisa Pacor, Roberto Corrocher, Claudio Lunardi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective. To investigate the T cell receptor (TCR) repertoire in psoriatic synovitis and to determine whether T lymphocytes in joint and skin lesions show the same Vβ CDR3 region. Methods. The expression of Vα and Vβ families was evaluated by reverse transcriptase-polymerase chain reaction. The CDR3 region of some Vβ families was analyzed by cloning and sequencing. Results. We found a diverse variable β chain usage within psoriatic synovial fluid of 11 patients although some Vα and Vβ families were more frequently expressed without evidence of clonality. Analysis of TCR in skin and synovial lesions of 3 patients showed identical CDR3 sequences, indicating that T cells bearing the same TCR are present at the 2 sites of chronic inflammation. Conclusion. These data suggest that common or similar crossreactive antigens present in the 2 locations are responsible for the expansion of the same TCR-bearing T cells possibly already activated by a superantigen. This supports the hypothesis that both polyclonal and oligoclonal lymphocyte activation contribute to the initiation and persistence of psoriatic arthritis.

Original languageEnglish
Pages (from-to)1914-1919
Number of pages6
JournalJournal of Rheumatology
Volume29
Issue number9
Publication statusPublished - Sep 2002

Fingerprint

Synovitis
T-Cell Antigen Receptor
Joints
T-Lymphocytes
Skin
Superantigens
Psoriatic Arthritis
Synovial Fluid
Lymphocyte Activation
Reverse Transcriptase Polymerase Chain Reaction
Organism Cloning
Inflammation
Antigens

Keywords

  • CDR3
  • Psoriatic arthritis
  • Superantigen
  • T cell receptor

ASJC Scopus subject areas

  • Rheumatology
  • Immunology

Cite this

The T cell receptor repertoire in psoriatic synovitis is restricted and T lymphocytes expressing the same TCR are present in joint and skin lesions. / Borgato, Lorena; Puccetti, Antonio; Beri, Ruggero; Codella, Orazio; Frigo, Antonio; Simeoni, Sara; Pacor, Maria Luisa; Corrocher, Roberto; Lunardi, Claudio.

In: Journal of Rheumatology, Vol. 29, No. 9, 09.2002, p. 1914-1919.

Research output: Contribution to journalArticle

Borgato, L, Puccetti, A, Beri, R, Codella, O, Frigo, A, Simeoni, S, Pacor, ML, Corrocher, R & Lunardi, C 2002, 'The T cell receptor repertoire in psoriatic synovitis is restricted and T lymphocytes expressing the same TCR are present in joint and skin lesions', Journal of Rheumatology, vol. 29, no. 9, pp. 1914-1919.
Borgato, Lorena ; Puccetti, Antonio ; Beri, Ruggero ; Codella, Orazio ; Frigo, Antonio ; Simeoni, Sara ; Pacor, Maria Luisa ; Corrocher, Roberto ; Lunardi, Claudio. / The T cell receptor repertoire in psoriatic synovitis is restricted and T lymphocytes expressing the same TCR are present in joint and skin lesions. In: Journal of Rheumatology. 2002 ; Vol. 29, No. 9. pp. 1914-1919.
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AU - Frigo, Antonio

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