The Targeted Delivery of Interleukin 4 Inhibits Development of Endometriotic Lesions in a Mouse Model

Federica Quattrone, Ana Maria Sanchez, Maria Pannese, Teresa Hemmerle, Paola Viganò, Massimo Candiani, Felice Petraglia, Dario Neri, Paola Panina-Bordignon

Research output: Contribution to journalArticlepeer-review

Abstract

Endometriosis is caused by the displacement of endometrium outside the uterus contributing heavily to infertility and debilitating pelvic pain. Ectopic adhesion and growth are believed to occur under the influence of a favorable hormonal environment and immunological factors. The objective of this study is to analyze the effect of a targeted therapy with an antibody-based pharmacodelivery of interleukin 4 (F8-IL4) in a mouse model of experimentally induced endometriosis. Endometriosis-like lesions were induced in Balb/c mice. The animals were treated intravenously with F8-IL4 or with untargeted IL4 (KSF-IL4). Twelve days after disease induction, the lesions were isolated. A significant reduction in the number of total lesions/mouse and in the total volume of lesions/mouse was observed in mice treated with F8-IL4 compared to controls (P =.029 and P =.006, respectively), while no difference was found between KSF-IL4-treated mice and their controls. Gene expression was evaluated by quantitative real-time polymerase chain reaction. Expression of genes involved in cell adhesion, extracellular matrix invasion, and neovascularization was significantly downregulated in F8-IL4-treated mice compared to their controls (integrin β1: P =.02; metalloproteinase [MMP] 3: P =.02; MMP9: P =.04; vascular endothelial growth factor: P =.04). Gene expression of inflammatory cytokines (tumor necrosis factor α, IL1β, IL1α, and IL6) did not vary in the ectopic lesions isolated from F8-IL4-treated mice compared to their controls. Immunohistochemistry demonstrated a significantly reduced expression of E-cadherin and β-catenin in the lesions of mice treated with F8-IL4. Our results show that the antibody-mediated targeted delivery of IL4 inhibits the development of endometriosis in a syngeneic mouse model by likely impairing adhesion, invasion, and vascularization of the ectopic endometrium.

Original languageEnglish
Pages (from-to)1143-1152
Number of pages10
JournalReproductive Sciences
Volume22
Issue number9
DOIs
Publication statusPublished - Sep 12 2015

Keywords

  • endometriosis
  • IL-4
  • immunocytokine

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Fingerprint Dive into the research topics of 'The Targeted Delivery of Interleukin 4 Inhibits Development of Endometriotic Lesions in a Mouse Model'. Together they form a unique fingerprint.

Cite this