The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence

Romina Burla, Mariateresa Carcuro, Mattia La Torre, Federica Fratini, Marco Crescenzi, M. R. D'Apice, Paola Spitalieri, Grazia D. Raffa, Letizia Astrologo, Giovanna Lattanzi, Enrico Cundari, Domenico Raimondo, Annamaria Biroccio, Maurizio Gatti, Isabella Saggio

Research output: Contribution to journalArticle

Abstract

AKTIP is a shelterin-interacting protein required for replication of telomeric DNA. Here, we show that AKTIP biochemically interacts with A- and B-type lamins and affects lamin A, but not lamin C or B, expression. In interphase cells, AKTIP localizes at the nuclear rim and in discrete regions of the nucleoplasm just like lamins. Double immunostaining revealed that AKTIP partially co-localizes with lamin B1 and lamin A/C in interphase cells, and that proper AKTIP localization requires functional lamin A. In mitotic cells, AKTIP is enriched at the spindle poles and at the midbody of late telophase cells similar to lamin B1. AKTIP-depleted cells show senescence-associated markers and recapitulate several aspects of the progeroid phenotype. Collectively, our results indicate that AKTIP is a new player in lamin-related processes, including those that govern nuclear architecture, telomere homeostasis and cellular senescence.

Original languageEnglish
Article number160103
JournalOpen Biology
Volume6
Issue number8
DOIs
Publication statusPublished - Aug 1 2016

Keywords

  • Cell senescence
  • Lamin
  • Laminopathies
  • Nuclear lamina
  • Progeria
  • Telomeres

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Burla, R., Carcuro, M., La Torre, M., Fratini, F., Crescenzi, M., D'Apice, M. R., Spitalieri, P., Raffa, G. D., Astrologo, L., Lattanzi, G., Cundari, E., Raimondo, D., Biroccio, A., Gatti, M., & Saggio, I. (2016). The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence. Open Biology, 6(8), [160103]. https://doi.org/10.1098/rsob.160103