The thrombin generation assay distinguishes inhibitor from non-inhibitor patients with severe haemophilia A

M. E. Mancuso, V. Chantarangkul, M. Clerici, M. R. Fasulo, L. Padovan, E. Scalambrino, F. Peyvandi, A. Tripodi, E. Santagostino

Research output: Contribution to journalArticlepeer-review

Abstract

Introduction: Patients with haemophilia A (HA) have impaired thrombin generation (TG) capacity and TG assay (TGA) values are linearly related to plasma factor VIII (FVIII) levels. Aim: This study carried out in patients with unmeasurable FVIII (−1) was aimed at unravelling any difference in TG capacity in patients with or without inhibitors. Methods: Blood samples were collected from patients in a non-bleeding state, after a 5-day wash-out period from last treatment. Results: TGA was performed in 102 patients with severe HA (15% with high-responding inhibitors; 51% with null F8 mutations, that as expected were more prevalent in inhibitor than in non-inhibitor patients). TG capacity was significantly lower in inhibitor than non-inhibitor patients and in those with null mutations than in those with non-null mutations. When the TG capacity was evaluated only in patients with null mutations with and without inhibitors it was lower in the presence of inhibitors. Conclusions: This study shows a greater TG impairment in inhibitor patients irrespective of FVIII levels, inhibitor titre and F8 mutation type, suggesting a role for the TGA in unravelling functional interferences of anti-FVIII inhibitors on coagulation system activation.

Original languageEnglish
Pages (from-to)e286-e291
JournalHaemophilia
Volume22
Issue number4
DOIs
Publication statusPublished - Jul 1 2016

Keywords

  • endogenous thrombin potential
  • F8 mutations
  • inhibitors
  • severe haemophilia A
  • thrombin generation assay

ASJC Scopus subject areas

  • Hematology
  • Genetics(clinical)

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