The Toxoplasma gondii bradyzoite antigens BAG1 and MAG1 induce early humoral and cell-mediated immune responses upon human infection

Manlio Di Cristina, Paola Del Porto, Wilma Buffolano, Elisa Beghetto, Andrea Spadoni, Silvia Guglietta, Enza Piccolella, Franco Felici, Nicola Gargano

Research output: Contribution to journalArticle

Abstract

Infection of humans by Toxoplasma gondii leads to an acute systemic phase, in which tachyzoites disseminate throughout the body, followed by a chronic phase characterized by the presence of tissue cysts, containing bradyzoites, in brain, heart and skeletal muscles. This work focused on studying the antigenic regions of bradyzoite-specific proteins involved in human B- and T-cell responses. To this aim, we constructed a phage-display library of DNA fragments derived from the bradyzoite-specific genes BAG1, MAG1, SAG2D, SAG4, BSR4, LDH2, ENO1 and p-ATPase. Challenge of the bradyzoite library with sera of infected individuals led to the identification of antigenic regions within BAG1 and MAG1 gene products. Analysis of the humoral and lymphoproliferative responses to recombinant antigens demonstrated that the BAG1 fragment induced T-cell proliferation in 34% of T. gondii-exposed individuals, while 50% of them had specific IgG. In the same subjects, the MAG1 fragment was recognized by T cells from 17% of the exposed donors and by antibodies from 73% of them. A detailed analysis of the antibody response against BAG1 and MAG1 antigen fragments demonstrated that the immune response against bradyzoites occurs early after infection in humans. Finally, we provide evidence that the T-cell response against BAG1 is associated with the production of interferon-gamma, suggesting that bradyzoite antigens should be considered in the design of potential vaccines in humans.

Original languageEnglish
Pages (from-to)164-171
Number of pages8
JournalMicrobes and Infection
Volume6
Issue number2
DOIs
Publication statusPublished - Feb 2004

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Toxoplasma
T-Lymphocytes
Antigens
Infection
Toxoplasmosis
Gene Library
Bacteriophages
Genes
Libraries
Interferon-gamma
Antibody Formation
Adenosine Triphosphatases
Cysts
Myocardium
Skeletal Muscle
Vaccines
Immunoglobulin G
Cell Proliferation
Antibodies
Brain

Keywords

  • Bradyzoite
  • Immunity
  • Phage-display
  • Recombinant antigens
  • Toxoplasma

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

Di Cristina, M., Del Porto, P., Buffolano, W., Beghetto, E., Spadoni, A., Guglietta, S., ... Gargano, N. (2004). The Toxoplasma gondii bradyzoite antigens BAG1 and MAG1 induce early humoral and cell-mediated immune responses upon human infection. Microbes and Infection, 6(2), 164-171. https://doi.org/10.1016/j.micinf.2003.11.009

The Toxoplasma gondii bradyzoite antigens BAG1 and MAG1 induce early humoral and cell-mediated immune responses upon human infection. / Di Cristina, Manlio; Del Porto, Paola; Buffolano, Wilma; Beghetto, Elisa; Spadoni, Andrea; Guglietta, Silvia; Piccolella, Enza; Felici, Franco; Gargano, Nicola.

In: Microbes and Infection, Vol. 6, No. 2, 02.2004, p. 164-171.

Research output: Contribution to journalArticle

Di Cristina, M, Del Porto, P, Buffolano, W, Beghetto, E, Spadoni, A, Guglietta, S, Piccolella, E, Felici, F & Gargano, N 2004, 'The Toxoplasma gondii bradyzoite antigens BAG1 and MAG1 induce early humoral and cell-mediated immune responses upon human infection', Microbes and Infection, vol. 6, no. 2, pp. 164-171. https://doi.org/10.1016/j.micinf.2003.11.009
Di Cristina, Manlio ; Del Porto, Paola ; Buffolano, Wilma ; Beghetto, Elisa ; Spadoni, Andrea ; Guglietta, Silvia ; Piccolella, Enza ; Felici, Franco ; Gargano, Nicola. / The Toxoplasma gondii bradyzoite antigens BAG1 and MAG1 induce early humoral and cell-mediated immune responses upon human infection. In: Microbes and Infection. 2004 ; Vol. 6, No. 2. pp. 164-171.
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