The transcriptome and miRNome profiling of glioblastoma tissues and peritumoral regions highlights molecular pathways shared by tumors and surrounding areas and reveals differences between short-term and long-term survivors

Barbara Fazi, Armando Felsani, Luigi Grassi, Anna Moles, Daniel D'Andrea, Nicola Toschi, Daria Sicari, Pasquale D. De Bonis, Carmelo Anile, Maria Giovanna Guerrisi, Emilia Luca, Maria Giulia Farace, Giulio Maira, Silvia Anna Ciafré, Annunziato Mangiola

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Glioblastoma multiforme (GBM) is the most common and deadliest primary brain tumor, driving patients to death within 15 months after diagnosis (short term survivors, ST), with the exception of a small fraction of patients (long term survivors, LT) surviving longer than 36 months. Here we present deep sequencing data showing that peritumoral (P) areas differ from healthy white matter, but share with their respective frankly tumoral (C) samples, a number of mRNAs and microRNAs representative of extracellular matrix remodeling, TGFß and signaling, of the involvement of cell types different from tumor cells but contributing to tumor growth, such as microglia or reactive astrocytes. Moreover, we provide evidence about RNAs differentially expressed in ST vs LT samples, suggesting the contribution of TGF-β signaling in this distinction too. We also show that the edited form of miR- 376c-3p is reduced in C vs P samples and in ST tumors compared to LT ones. As a whole, our study provides new insights into the still puzzling distinction between ST and LT tumors, and sheds new light onto that "grey" zone represented by the area surrounding the tumor, which we show to be characterized by the expression of several molecules shared with the proper tumor mass.

Original languageEnglish
Pages (from-to)22526-22552
Number of pages27
JournalOncotarget
Volume6
Issue number26
Publication statusPublished - 2015

Fingerprint

Gene Expression Profiling
Glioblastoma
Survivors
Neoplasms
High-Throughput Nucleotide Sequencing
Microglia
MicroRNAs
Brain Neoplasms
Astrocytes
Extracellular Matrix
RNA
Messenger RNA
Growth

Keywords

  • Editing
  • Glioblastoma
  • MicroRNA
  • Peritumoral area
  • TGFß

ASJC Scopus subject areas

  • Oncology

Cite this

The transcriptome and miRNome profiling of glioblastoma tissues and peritumoral regions highlights molecular pathways shared by tumors and surrounding areas and reveals differences between short-term and long-term survivors. / Fazi, Barbara; Felsani, Armando; Grassi, Luigi; Moles, Anna; D'Andrea, Daniel; Toschi, Nicola; Sicari, Daria; De Bonis, Pasquale D.; Anile, Carmelo; Guerrisi, Maria Giovanna; Luca, Emilia; Farace, Maria Giulia; Maira, Giulio; Ciafré, Silvia Anna; Mangiola, Annunziato.

In: Oncotarget, Vol. 6, No. 26, 2015, p. 22526-22552.

Research output: Contribution to journalArticle

Fazi, B, Felsani, A, Grassi, L, Moles, A, D'Andrea, D, Toschi, N, Sicari, D, De Bonis, PD, Anile, C, Guerrisi, MG, Luca, E, Farace, MG, Maira, G, Ciafré, SA & Mangiola, A 2015, 'The transcriptome and miRNome profiling of glioblastoma tissues and peritumoral regions highlights molecular pathways shared by tumors and surrounding areas and reveals differences between short-term and long-term survivors', Oncotarget, vol. 6, no. 26, pp. 22526-22552.
Fazi, Barbara ; Felsani, Armando ; Grassi, Luigi ; Moles, Anna ; D'Andrea, Daniel ; Toschi, Nicola ; Sicari, Daria ; De Bonis, Pasquale D. ; Anile, Carmelo ; Guerrisi, Maria Giovanna ; Luca, Emilia ; Farace, Maria Giulia ; Maira, Giulio ; Ciafré, Silvia Anna ; Mangiola, Annunziato. / The transcriptome and miRNome profiling of glioblastoma tissues and peritumoral regions highlights molecular pathways shared by tumors and surrounding areas and reveals differences between short-term and long-term survivors. In: Oncotarget. 2015 ; Vol. 6, No. 26. pp. 22526-22552.
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