The two C/EBP isoforms, IL-6DBP/NF-IL6 and C/EBPδ/NF-IL6β, are induced by IL-6 to promote acute phase gene transcription via different mechanisms

D. P. Ramji, A. Vitelli, F. Tronche, R. Cortese, G. Ciliberto

Research output: Contribution to journalArticle

Abstract

The promoter regions of three IL-6 inducible genes, hemopexin (Hpx), haptoglobin (Hp) and C-reactive protein (CRP) contain cis-acting IL-6 responsive elements (IL-6REs) which are necessary and sufficient to induce IL-6 transcription activation. Transcription factors of the C/EBP family interact with IL-6RES. Among these, IL-6DBP/NF-IL6 plays a key role in IL-6 signal transduction because its trans-activation potential is induced by IL-6 in the human hepatoma cell line Hep3B. We show here that a different C/EBP-related factor, C/EBPδ/NF-IL6β, is the major IL-6 induced protein interacting with IL-6REs in the nuclei of Hep3B cells. In contrast to IL-6DBP/NF-IL6, whose activity in Hep3B cells is modulated by IL-6 via a post-translational mechanism, C/EBPδ/NF-IL6β is transcriptionally induced by IL-6. Another contrasting feature is that the C/EBPδ cDNA transfected in Hep3B cells activates transcription from an IL-6RE synthetic promoter in a constitutive manner which is not further enhanced by IL-6. Therefore, in Hep3B cells, two distinct members of the C/EBP family are recruited in the IL-6 signal transduction pathway via different mechanisms.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalNucleic Acids Research
Volume21
Issue number2
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

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