The two phyto-oestrogens genistein and quercetin exert different effects on oestrogen receptor function

P. Miodini, L. Fioravanti, G. Di Fronzo, V. Cappelletti

Research output: Contribution to journalArticle

Abstract

We compared the oestrogenic and anti-oestrogenic properties of the two well-known phyto-oestrogens, genistein and quercetin, on the oestrogen-sensitive breast cancer cell line MCF-7. Genistein exerted a biphasic effect on growth of MCF-7 cells, stimulating at low and inhibiting at high concentrations, whereas quercetin was only growth inhibitory. At doses which did not inhibit cell growth, respectively 5 and 1 μM, genistein and quercetin counteracted oestrogen- and transforming growth factor-α-promoted cell growth stimulation. Furthermore, genistein promoted transcription of the oestrogen-regulated genes pS2 and cathepsin-D, whereas quercetin interfered with the oestrogen-induced expression of the proteins. In in vitro binding experiments, genistein competed with oestradiol for binding to the oestrogen receptor (ER), but quercetin did not. Quercetin and genistein down-regulated cytoplasmic ER levels and promoted a tighter nuclear association of the ER, but only genistein was able to up-regulate progesterone receptor protein levels. In gel mobility assays, ER preincubation with oestradiol or with the two phyto-oestrogens led to the appearance of the same retarded band, excluding differences between the various complexes in binding to the consensus sequence. The data allowed us to conclude that quercetin acts like a pure anti-oestrogen, whereas genistein displays mixed agonist/antagonist properties, and to formulate a hypothesis on the possible mechanism of action of such phyto-oestrogens.

Original languageEnglish
Pages (from-to)1150-1155
Number of pages6
JournalBritish Journal of Cancer
Volume80
Issue number8
DOIs
Publication statusPublished - 1999

Fingerprint

Phytoestrogens
Genistein
Quercetin
Estrogen Receptors
Estrogens
Growth
Estradiol
Cathepsin D
MCF-7 Cells
Consensus Sequence
Transforming Growth Factors
Progesterone Receptors
Proteins
Up-Regulation
Gels
Breast Neoplasms
Cell Line

Keywords

  • Breast cancer
  • Genistein
  • Oestrogen receptor
  • Phyto-oestrogens
  • Quercetin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

The two phyto-oestrogens genistein and quercetin exert different effects on oestrogen receptor function. / Miodini, P.; Fioravanti, L.; Di Fronzo, G.; Cappelletti, V.

In: British Journal of Cancer, Vol. 80, No. 8, 1999, p. 1150-1155.

Research output: Contribution to journalArticle

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