The type II decoy receptor of IL-1 inhibits murine collagen-induced arthritis

Natacha Bessis, Laurence Guéry, Alberto Mantovani, Annunciata Vecchi, John E. Sims, Didier Fradelizi, Marie Christophe Boissier

Research output: Contribution to journalArticlepeer-review

Abstract

IL-1 is a key cytokine involved in the inflammatory response. The type II receptor of IL-1 (IL-1RII) acts as a decoy receptor, binding and inhibiting the effect of IL-1. This study was undertaken to establish whether IL-1RII can ameliorate collagen-induced arthritis, a model of inflammatory arthritis in mice. We used human keratinocytes transfected with the human (h)lL-1RII gene as a source of hIL-1RII protein. We showed that these cells expressed both the membrane and soluble form of receptor. In vitro, IL-1-stimulated murine macrophage cells showed a decreased expression of TNF-α in the presence of hIL-1RII. We engrafted the hIL-1RII-transfected cells in the back of mice developing collagen-induced arthritis. We found that clinical and histological parameters of arthritis were significantly decreased in mice treated with cells producing hIL-1RII. In addition, hIL-1RII administration was able to reduce the expression of mRNA for IL-6 and myeloperoxidase in the joints of treated animals. These data show that hIL-1RII anti-inflammatory properties in the model of collagen-induced arthritis in mice and could have a regulatory role in rheumatoid arthritis.

Original languageEnglish
Pages (from-to)867-875
Number of pages9
JournalEuropean Journal of Immunology
Volume30
Issue number3
DOIs
Publication statusPublished - 2000

Keywords

  • Cytokine
  • Gene therapy
  • In vivo animal model
  • Inflammation
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Immunology

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