The tyrosine phosphatase HD-PTP: A novel player in endothelial migration

Sara Castiglioni, Jeanette A M Maier, Massimo Mariotti

Research output: Contribution to journalArticle

Abstract

Endothelial migration, pivotal step of angiogenesis, is tightly tuned by tyrosine phosphorylation of different substrates, which results from the coordinated action of tyrosine kinases and phosphatases. Here we report that the tyrosine phosphatase HD-PTP has a role in modulating endothelial motility. Indeed, we found that endothelial cells downregulating HD-PTP after transfection with siRNA acquire a scattered and spindle-shaped phenotype and migrate more than controls. We also show that HD-PTP binds Focal Adhesion Kinase (FAK), a crucial regulator of cell migration. This interaction is strongly inhibited by treatment with basic Fibroblast Growth Factor, an angiogenic factor which stimulates endothelial cell migration. In cells downregulating HD-PTP, FAK is hyperphosphorylated on tyrosine residues and localizes in the focal adhesions, at the leading edge of the cell. We suggest that HD-PTP contributes to the regulation of endothelial motility by modulating the tyrosine phosphorylation of FAK.

Original languageEnglish
Pages (from-to)534-539
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume364
Issue number3
DOIs
Publication statusPublished - Dec 21 2007

Keywords

  • Cell migration
  • Endothelium
  • FAK
  • HD-PTP

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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