The tyrosine phosphatase HD-PTP is regulated by FGF-2 through proteasome degradation

Massimo Mariotti, Sara Castiglioni, Laura Beguinot, Jeanette A M Maier

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Angiogenesis is essential in development and wound healing and contributes to the pathogenesis of many diseases. The signalling pathways activated in angiogenesis are, in part, known and the overall tyrosine phosphorylation of cellular proteins plays a relevant role. By RNA fingerprinting, we isolated a tyrosine phosphatase, HD-PTP, modulated in human endothelial cells exposed to human immunodeficiency virus type 1 Tat, a viral protein known to be angiogenic. For the first time, we describe HD-PTP at the protein level. HD-PTP, a 185 kDa cytosolic protein which is expressed in endothelial cells of different origin. We show that HD-PTP is upregulated by Tat at the mRNA but not at the protein level. HD-PTP protein is differentially modulated by two angiogenic growth factors. While Vascular Endothelial Growth Factor does not affect protein levels, Fibroblast Growth Factor-2 induces HD-PTP degradation via the proteasome system.

Original languageEnglish
Pages (from-to)2138-2143
Number of pages6
JournalFrontiers in Bioscience
Volume11
Issue numberSUPPL. 1
DOIs
Publication statusPublished - 2006

Fingerprint

Fibroblast Growth Factor 2
Proteasome Endopeptidase Complex
Phosphoric Monoester Hydrolases
Tyrosine
Degradation
Proteins
Endothelial cells
Endothelial Cells
Phosphorylation
Angiogenesis Inducing Agents
Viral Proteins
Viruses
Wound Healing
Vascular Endothelial Growth Factor A
HIV-1
Intercellular Signaling Peptides and Proteins
RNA
Messenger RNA

Keywords

  • Angiogenesis
  • Fibroblast Growth Factor
  • HD-PTP
  • HIV-1-Tat
  • Proteasome
  • Vascular Endothelial Growth Factor
  • Vessel

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

The tyrosine phosphatase HD-PTP is regulated by FGF-2 through proteasome degradation. / Mariotti, Massimo; Castiglioni, Sara; Beguinot, Laura; Maier, Jeanette A M.

In: Frontiers in Bioscience, Vol. 11, No. SUPPL. 1, 2006, p. 2138-2143.

Research output: Contribution to journalArticle

Mariotti, Massimo ; Castiglioni, Sara ; Beguinot, Laura ; Maier, Jeanette A M. / The tyrosine phosphatase HD-PTP is regulated by FGF-2 through proteasome degradation. In: Frontiers in Bioscience. 2006 ; Vol. 11, No. SUPPL. 1. pp. 2138-2143.
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