The use of β-galactosidase as a tracer in immunocytochemistry

A. Bondi, G. Chieregatti, V. Eusebi, E. Fulcheri, G. Bussolati

Research output: Contribution to journalArticle

Abstract

A new immunocytochemical method using β-galactosidase as a tracer is described. The positive staining appears blue on an unstained background. The present method has the high sensitivity and specificity of the immunoperoxidase method and appears to be a practical alternative. The substrate has no carcinogenic activity. Staining is permanent and the sections can be dehydrated and mounted in synthetic media. Enzyme and substrate solutions are stable for several months.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalHistochemistry
Volume76
Issue number2
DOIs
Publication statusPublished - Jun 1982

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Galactosidases
Immunohistochemistry
Staining and Labeling
Sensitivity and Specificity
Enzymes

ASJC Scopus subject areas

  • Anatomy

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Bondi, A., Chieregatti, G., Eusebi, V., Fulcheri, E., & Bussolati, G. (1982). The use of β-galactosidase as a tracer in immunocytochemistry. Histochemistry, 76(2), 153-158. https://doi.org/10.1007/BF00501918

The use of β-galactosidase as a tracer in immunocytochemistry. / Bondi, A.; Chieregatti, G.; Eusebi, V.; Fulcheri, E.; Bussolati, G.

In: Histochemistry, Vol. 76, No. 2, 06.1982, p. 153-158.

Research output: Contribution to journalArticle

Bondi, A, Chieregatti, G, Eusebi, V, Fulcheri, E & Bussolati, G 1982, 'The use of β-galactosidase as a tracer in immunocytochemistry', Histochemistry, vol. 76, no. 2, pp. 153-158. https://doi.org/10.1007/BF00501918
Bondi, A. ; Chieregatti, G. ; Eusebi, V. ; Fulcheri, E. ; Bussolati, G. / The use of β-galactosidase as a tracer in immunocytochemistry. In: Histochemistry. 1982 ; Vol. 76, No. 2. pp. 153-158.
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