The use of pronase enhances sensitivity of the PAP method in the detection of intracytoplasmic immunoglobulins

S. Pileri, L. Serra, G. Martinelli

Research output: Contribution to journalArticle

Abstract

Application of immunocytochemistry to formalin-fixed, paraffin-embedded material tends to provide capricious results, even when employing the unlablleled antibody peroxidase-antiperoxidase (PAP) method. This study shows that treatment of tissue sections with pronase, a protease isolated from a strain of Streptomyces griseus, prior sections with pronase, a protease isolated from a strain of Streptomyces griseus, prior to performance of the PAP procedure greatly enhances its sensitivity. The optimum conditions for use of this enzyme in detecting intracellular immunoglobulins are given.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalBasic and Applied Histochemistry
Volume24
Issue number3
Publication statusPublished - 1980

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ASJC Scopus subject areas

  • Anatomy

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