The use of quantitative real time polymerase chain reaction to quantify some rumen bacterial strains in an in vitro rumen system

Lucy Onime, Cristina Zanfi, Chiara Agostinis, Roberta Bulla, Mauro Spanghero

Research output: Contribution to journalArticle

Abstract

The aim of this work was to quantify four rumen bacterial strains (Butyrivibrio fibrisolvens, Ruminococcus albus, Streptococcus bovis, Megasphaera elsdenii) in an in vitro batch rumen fermentative system by quantitative real time polymerase chain reaction (qPCR). The experiment was a 2×2 factorial arrangement with two types of liquid rumen, collected from dairy cows (DC) and fattening bulls (FB) and two types of fermentation substrate (forage:concentrate ratios, 75:25 and 25:75) and was replicated in two fermentation runs. Fermentation fluids from FB compared to those from DC had lower pH, higher total VFA concentrations (averages of 0 and 24 h samplings, 6.70 vs 7.04 and 72.6 vs 42.7 mmol/l P9 copies /mL), while M. elsdenii was detected only in FB fermentation fluids. R. albus and S. bovisconcentrations were not different between the two types of rumen liquid. With the only exception for B. fibrosolvens, bacteria strains considered in this study increased their concentrations in the fermentation fluid during the 24 h of in vitro incubation.

Original languageEnglish
Pages (from-to)366-370
Number of pages5
JournalItalian Journal of Animal Science
Volume12
Issue number3
DOIs
Publication statusPublished - 2013

Fingerprint

rumen
quantitative polymerase chain reaction
fermentation
Megasphaera elsdenii
Ruminococcus albus
finishing
bulls
dairy cows
Butyrivibrio fibrisolvens
Streptococcus bovis
liquids
concentrates
forage
bacteria
fluids
sampling

Keywords

  • Bacteria
  • In vitro fermentation
  • qPCR
  • Rumen

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

The use of quantitative real time polymerase chain reaction to quantify some rumen bacterial strains in an in vitro rumen system. / Onime, Lucy; Zanfi, Cristina; Agostinis, Chiara; Bulla, Roberta; Spanghero, Mauro.

In: Italian Journal of Animal Science, Vol. 12, No. 3, 2013, p. 366-370.

Research output: Contribution to journalArticle

Onime, Lucy ; Zanfi, Cristina ; Agostinis, Chiara ; Bulla, Roberta ; Spanghero, Mauro. / The use of quantitative real time polymerase chain reaction to quantify some rumen bacterial strains in an in vitro rumen system. In: Italian Journal of Animal Science. 2013 ; Vol. 12, No. 3. pp. 366-370.
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