The V1/V2 loop of HIV-1 gp120 is necessary for Tat binding and consequent modulation of virus entry

Sabrina Cardaci, Marco Soster, Federico Bussolino, Serena Marchiò

Research output: Contribution to journalArticle

Abstract

Preventing cell entry of human immunodeficiency virus 1 (HIV-1) is of interest for the development of innovative therapies. We previously reported a specific interaction between HIV-1 envelope glycoprotein 120 (gp120) and Tat at the cell surface, which enhances virus attachment and entry. We also identified a gp120-mimicking peptide, CT319, that competes with gp120 for Tat binding, thus inhibiting HIV-1 infection. Here we report a molecular dissection of gp120 regions involved in this mechanism. Our findings identify the V1/V2 loop of gp120 as involved in Tat binding, and define this interaction as functionally relevant for HIV-1 entry into host cells.

Original languageEnglish
Pages (from-to)2943-2951
Number of pages9
JournalFEBS Letters
Volume587
Issue number18
DOIs
Publication statusPublished - Sep 17 2013

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Keywords

  • gp120
  • HIV-1
  • Peptide
  • Tat
  • Virus entry

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

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