The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma

Andrea di Cristofori, Stefano Ferrero, Irene Bertolini, Gabriella Gaudioso, Maria Veronica Russo, Valeria Berno, Marco Vanini, Marco Locatelli, Mario Zavanone, Paolo Rampini, Thomas Vaccari, Manuela Caroli, Valentina Vaira

Research output: Contribution to journalArticle

Abstract

The vacuolar H+ ATPase (V-ATPase) is a proton pump responsible for acidification of cellular microenvironments, an activity exploited by tumors to survive, proliferate and resist to therapy. Despite few observations, the role of V-ATPase in human tumorigenesis remains unclear. We investigated the expression of ATP6V0C, ATP6V0A2, encoding two subunits belonging to the V-ATPase V0 sector and ATP6V1C, ATP6V1G1, ATPT6V1G2, ATP6V1G3, which are part of the V1 sector, in series of adult gliomas and in cancer stem cell-enriched neurospheres isolated from glioblastoma (GBM) patients. ATP6V1G1 expression resulted significantly upregulated in tissues of patients with GBM and correlated with shorter patients' overall survival independent of clinical variables. ATP6V1G1 knockdown in GBM neurospheres hampered sphere-forming ability, induced cell death, and decreased matrix invasion, a phenotype not observed in GBM monolayer cultures. Treating GBM organotypic cultures or neurospheres with the selective V-ATPase inhibitor bafilomycin A1 reproduced the effects of ATP6V1G1 siRNA and strongly suppressed expression of the stem cell markers Nestin, CD133 and transcription factors SALL2 and POU3F2 in neurospheres. These data point to ATP6V1G1 as a novel marker of poor prognosis in GBM patients and identify V-ATPase inhibition as an innovative therapeutic strategy for GBM.

Original languageEnglish
Pages (from-to)17514-17531
Number of pages18
JournalOncotarget
Volume6
Issue number19
Publication statusPublished - 2015

Keywords

  • Bafilomycin A1
  • Cancer stem cells
  • Glioblastoma
  • Organotypic tissue cultures
  • Vacuolar H+-ATPase

ASJC Scopus subject areas

  • Oncology

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    di Cristofori, A., Ferrero, S., Bertolini, I., Gaudioso, G., Russo, M. V., Berno, V., Vanini, M., Locatelli, M., Zavanone, M., Rampini, P., Vaccari, T., Caroli, M., & Vaira, V. (2015). The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma. Oncotarget, 6(19), 17514-17531.