Abstract
The assumption of molecular epidemiology that carcinogens leave fingerprints has suggested that analysis of the frequency, type, and site of mutations in genes frequently altered in carcinogenesis may provide clues to the identification of the factors contributing to carcinogenesis. In this mini-review, we revise the development, and validation of the yeast-based p53 functional assay as a new tool for molecular epidemiology. We show that this assay has some very interesting virtues but also has some drawbacks. The yeast functional assay can be used to determine highly specific mutation fingerprints in the human p53 cDNA sequence. Discrimination is possible when comparing mutation spectra induced by sufficiently different mutagens. However, we also reported that the same carcinogen may induce distinguishable mutation spectra due to known influencing factors. Copyright (C) 2000 Elsevier Science B.V.
| Original language | English |
|---|---|
| Pages (from-to) | 293-301 |
| Number of pages | 9 |
| Journal | Mutation Research - Reviews in Mutation Research |
| Volume | 462 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - Apr 2000 |
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Keywords
- Mutation spectra
- p53
- Yeast functional assay
ASJC Scopus subject areas
- Health, Toxicology and Mutagenesis
Cite this
The yeast p53 functional assay : A new tool for molecular epidemiology. Hopes and facts. / Fronza, Gilberto; Inga, Alberto; Monti, Paola; Scott, Gina; Campomenosi, Paola; Menichini, Paola; Ottaggio, Laura; Viaggi, Silvia; Burns, Philip A.; Gold, Barry; Abbondandolo, Angelo.
In: Mutation Research - Reviews in Mutation Research, Vol. 462, No. 2-3, 04.2000, p. 293-301.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The yeast p53 functional assay
T2 - A new tool for molecular epidemiology. Hopes and facts
AU - Fronza, Gilberto
AU - Inga, Alberto
AU - Monti, Paola
AU - Scott, Gina
AU - Campomenosi, Paola
AU - Menichini, Paola
AU - Ottaggio, Laura
AU - Viaggi, Silvia
AU - Burns, Philip A.
AU - Gold, Barry
AU - Abbondandolo, Angelo
PY - 2000/4
Y1 - 2000/4
N2 - The assumption of molecular epidemiology that carcinogens leave fingerprints has suggested that analysis of the frequency, type, and site of mutations in genes frequently altered in carcinogenesis may provide clues to the identification of the factors contributing to carcinogenesis. In this mini-review, we revise the development, and validation of the yeast-based p53 functional assay as a new tool for molecular epidemiology. We show that this assay has some very interesting virtues but also has some drawbacks. The yeast functional assay can be used to determine highly specific mutation fingerprints in the human p53 cDNA sequence. Discrimination is possible when comparing mutation spectra induced by sufficiently different mutagens. However, we also reported that the same carcinogen may induce distinguishable mutation spectra due to known influencing factors. Copyright (C) 2000 Elsevier Science B.V.
AB - The assumption of molecular epidemiology that carcinogens leave fingerprints has suggested that analysis of the frequency, type, and site of mutations in genes frequently altered in carcinogenesis may provide clues to the identification of the factors contributing to carcinogenesis. In this mini-review, we revise the development, and validation of the yeast-based p53 functional assay as a new tool for molecular epidemiology. We show that this assay has some very interesting virtues but also has some drawbacks. The yeast functional assay can be used to determine highly specific mutation fingerprints in the human p53 cDNA sequence. Discrimination is possible when comparing mutation spectra induced by sufficiently different mutagens. However, we also reported that the same carcinogen may induce distinguishable mutation spectra due to known influencing factors. Copyright (C) 2000 Elsevier Science B.V.
KW - Mutation spectra
KW - p53
KW - Yeast functional assay
UR - http://www.scopus.com/inward/record.url?scp=0034086025&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034086025&partnerID=8YFLogxK
U2 - 10.1016/S1383-5742(00)00011-9
DO - 10.1016/S1383-5742(00)00011-9
M3 - Article
C2 - 10767639
AN - SCOPUS:0034086025
VL - 462
SP - 293
EP - 301
JO - Mutation Research - Reviews in Mutation Research
JF - Mutation Research - Reviews in Mutation Research
SN - 1383-5742
IS - 2-3
ER -