The yeast p53 functional assay: A new tool for molecular epidemiology. Hopes and facts

Gilberto Fronza, Alberto Inga, Paola Monti, Gina Scott, Paola Campomenosi, Paola Menichini, Laura Ottaggio, Silvia Viaggi, Philip A. Burns, Barry Gold, Angelo Abbondandolo

Research output: Contribution to journalArticle

Abstract

The assumption of molecular epidemiology that carcinogens leave fingerprints has suggested that analysis of the frequency, type, and site of mutations in genes frequently altered in carcinogenesis may provide clues to the identification of the factors contributing to carcinogenesis. In this mini-review, we revise the development, and validation of the yeast-based p53 functional assay as a new tool for molecular epidemiology. We show that this assay has some very interesting virtues but also has some drawbacks. The yeast functional assay can be used to determine highly specific mutation fingerprints in the human p53 cDNA sequence. Discrimination is possible when comparing mutation spectra induced by sufficiently different mutagens. However, we also reported that the same carcinogen may induce distinguishable mutation spectra due to known influencing factors. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)293-301
Number of pages9
JournalMutation Research - Reviews in Mutation Research
Volume462
Issue number2-3
DOIs
Publication statusPublished - Apr 2000

Fingerprint

Hope
Molecular Epidemiology
Yeasts
Dermatoglyphics
Carcinogens
Mutation
Carcinogenesis
Mutagens
Mutation Rate
Complementary DNA
Genes

Keywords

  • Mutation spectra
  • p53
  • Yeast functional assay

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis

Cite this

The yeast p53 functional assay : A new tool for molecular epidemiology. Hopes and facts. / Fronza, Gilberto; Inga, Alberto; Monti, Paola; Scott, Gina; Campomenosi, Paola; Menichini, Paola; Ottaggio, Laura; Viaggi, Silvia; Burns, Philip A.; Gold, Barry; Abbondandolo, Angelo.

In: Mutation Research - Reviews in Mutation Research, Vol. 462, No. 2-3, 04.2000, p. 293-301.

Research output: Contribution to journalArticle

Fronza, G, Inga, A, Monti, P, Scott, G, Campomenosi, P, Menichini, P, Ottaggio, L, Viaggi, S, Burns, PA, Gold, B & Abbondandolo, A 2000, 'The yeast p53 functional assay: A new tool for molecular epidemiology. Hopes and facts', Mutation Research - Reviews in Mutation Research, vol. 462, no. 2-3, pp. 293-301. https://doi.org/10.1016/S1383-5742(00)00011-9
Fronza, Gilberto ; Inga, Alberto ; Monti, Paola ; Scott, Gina ; Campomenosi, Paola ; Menichini, Paola ; Ottaggio, Laura ; Viaggi, Silvia ; Burns, Philip A. ; Gold, Barry ; Abbondandolo, Angelo. / The yeast p53 functional assay : A new tool for molecular epidemiology. Hopes and facts. In: Mutation Research - Reviews in Mutation Research. 2000 ; Vol. 462, No. 2-3. pp. 293-301.
@article{2acf558007ca4b65a19894661fc59f98,
title = "The yeast p53 functional assay: A new tool for molecular epidemiology. Hopes and facts",
abstract = "The assumption of molecular epidemiology that carcinogens leave fingerprints has suggested that analysis of the frequency, type, and site of mutations in genes frequently altered in carcinogenesis may provide clues to the identification of the factors contributing to carcinogenesis. In this mini-review, we revise the development, and validation of the yeast-based p53 functional assay as a new tool for molecular epidemiology. We show that this assay has some very interesting virtues but also has some drawbacks. The yeast functional assay can be used to determine highly specific mutation fingerprints in the human p53 cDNA sequence. Discrimination is possible when comparing mutation spectra induced by sufficiently different mutagens. However, we also reported that the same carcinogen may induce distinguishable mutation spectra due to known influencing factors. Copyright (C) 2000 Elsevier Science B.V.",
keywords = "Mutation spectra, p53, Yeast functional assay",
author = "Gilberto Fronza and Alberto Inga and Paola Monti and Gina Scott and Paola Campomenosi and Paola Menichini and Laura Ottaggio and Silvia Viaggi and Burns, {Philip A.} and Barry Gold and Angelo Abbondandolo",
year = "2000",
month = "4",
doi = "10.1016/S1383-5742(00)00011-9",
language = "English",
volume = "462",
pages = "293--301",
journal = "Mutation Research - Reviews in Mutation Research",
issn = "1383-5742",
publisher = "Elsevier B.V.",
number = "2-3",

}

TY - JOUR

T1 - The yeast p53 functional assay

T2 - A new tool for molecular epidemiology. Hopes and facts

AU - Fronza, Gilberto

AU - Inga, Alberto

AU - Monti, Paola

AU - Scott, Gina

AU - Campomenosi, Paola

AU - Menichini, Paola

AU - Ottaggio, Laura

AU - Viaggi, Silvia

AU - Burns, Philip A.

AU - Gold, Barry

AU - Abbondandolo, Angelo

PY - 2000/4

Y1 - 2000/4

N2 - The assumption of molecular epidemiology that carcinogens leave fingerprints has suggested that analysis of the frequency, type, and site of mutations in genes frequently altered in carcinogenesis may provide clues to the identification of the factors contributing to carcinogenesis. In this mini-review, we revise the development, and validation of the yeast-based p53 functional assay as a new tool for molecular epidemiology. We show that this assay has some very interesting virtues but also has some drawbacks. The yeast functional assay can be used to determine highly specific mutation fingerprints in the human p53 cDNA sequence. Discrimination is possible when comparing mutation spectra induced by sufficiently different mutagens. However, we also reported that the same carcinogen may induce distinguishable mutation spectra due to known influencing factors. Copyright (C) 2000 Elsevier Science B.V.

AB - The assumption of molecular epidemiology that carcinogens leave fingerprints has suggested that analysis of the frequency, type, and site of mutations in genes frequently altered in carcinogenesis may provide clues to the identification of the factors contributing to carcinogenesis. In this mini-review, we revise the development, and validation of the yeast-based p53 functional assay as a new tool for molecular epidemiology. We show that this assay has some very interesting virtues but also has some drawbacks. The yeast functional assay can be used to determine highly specific mutation fingerprints in the human p53 cDNA sequence. Discrimination is possible when comparing mutation spectra induced by sufficiently different mutagens. However, we also reported that the same carcinogen may induce distinguishable mutation spectra due to known influencing factors. Copyright (C) 2000 Elsevier Science B.V.

KW - Mutation spectra

KW - p53

KW - Yeast functional assay

UR - http://www.scopus.com/inward/record.url?scp=0034086025&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034086025&partnerID=8YFLogxK

U2 - 10.1016/S1383-5742(00)00011-9

DO - 10.1016/S1383-5742(00)00011-9

M3 - Article

C2 - 10767639

AN - SCOPUS:0034086025

VL - 462

SP - 293

EP - 301

JO - Mutation Research - Reviews in Mutation Research

JF - Mutation Research - Reviews in Mutation Research

SN - 1383-5742

IS - 2-3

ER -