The zebrafish scale as model to study the bone mineralization process

Sara Pasqualetti, Giuseppe Banfi, Massimo Mariotti

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Danio rerio (zebrafish) shows high similarity with humans in terms of bone architecture, bone cells, matrix proteins and molecular signalling. The fish body is covered by elasmoid scales which are part of the dermal skeleton. Since few data have been published about the function of the fish scale cells, we investigated the mineralization pattern of the scale and the role of the episquamal osteoblasts in the neodeposition of the bone tissue. First, we described a specific mineralization pattern and distribution of the bone forming cells in different areas of the scale. We observed along the external circuli that, during the scale growth, the marginal cells migrate and organize in a cord-like structure just before the mineralization process takes place generating a new circulus. These cells exhibit alkaline phosphatase activity, a well known mammalian osteoblastic differentiation marker. The internal circuli are also characterized by new matrix deposition. Thus, zebrafish scale represents a useful model for analyzing the osteoblast behaviour during bone formation and mineralization and it could be useful in physiological studies and pharmacological tests.

Original languageEnglish
Pages (from-to)589-595
Number of pages7
JournalJournal of Molecular Histology
Volume43
Issue number5
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Physiologic Calcification
Zebrafish
Osteoblasts
Bone and Bones
Fishes
Bone Matrix
Differentiation Antigens
Osteogenesis
Skeleton
Alkaline Phosphatase
Pharmacology
Skin
Growth
Proteins

Keywords

  • Bone
  • Mineralization
  • Osteoblast
  • Scale
  • Zebrafish

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Physiology

Cite this

The zebrafish scale as model to study the bone mineralization process. / Pasqualetti, Sara; Banfi, Giuseppe; Mariotti, Massimo.

In: Journal of Molecular Histology, Vol. 43, No. 5, 10.2012, p. 589-595.

Research output: Contribution to journalArticle

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