Theophylline induced non specific suppressor activity in human peripheral blood lymphocytes

M. R. Zocchi, R. Pardi, G. Gromo, E. Ferrero, M. E. Ferrero, C. Besana, C. Rugarli

Research output: Contribution to journalArticle

Abstract

It is well known that theophylline yields phenotypic changes on suppressor cells. In the present study we investigated the possibility that theophylline could directly induce a suppressor activity on a lymphocyte subpopulation. We observed that a short preincubation (120 min at 37°C) with theophylline (1 mM) activates human peripheral blood lymphocytes to suppress mitogenic response of autologous cells. This activity was not evident on a T cell subpopulation depleted of theophylline-sensitive (T-sens) lymphocytes. Theophylline mediated suppressor activity is only present in the Concanavalin A stimulated cultures, thus suggesting a synergism between Concanavalin A and theophylline in the expression of non specific suppression. Moreover we observed that after a 24 hrs preincubation of lymphocytes in complete culture medium there was a complete loss of theophylline-induced suppression. Such a preincubation time also produced a decrease in the theophylline-mediated enhancement of intracellular 3',5' cyclic adenosine monophosphate levels and the impairment of E-rosette formation, suggesting that theophylline acts mainly on a 'short-lived' suppressor lymphocyte subset.

Original languageEnglish
Pages (from-to)217-234
Number of pages18
JournalJournal of Immunopharmacology
Volume7
Issue number2
Publication statusPublished - 1985

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Lymphocytes
Theophylline
Human Activities
Blood
Lymphocyte Subsets
Concanavalin A
Rosette Formation
T-cells
Cyclic AMP
Culture Media
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology
  • Toxicology
  • Pharmacology

Cite this

Theophylline induced non specific suppressor activity in human peripheral blood lymphocytes. / Zocchi, M. R.; Pardi, R.; Gromo, G.; Ferrero, E.; Ferrero, M. E.; Besana, C.; Rugarli, C.

In: Journal of Immunopharmacology, Vol. 7, No. 2, 1985, p. 217-234.

Research output: Contribution to journalArticle

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AU - Zocchi, M. R.

AU - Pardi, R.

AU - Gromo, G.

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AU - Besana, C.

AU - Rugarli, C.

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N2 - It is well known that theophylline yields phenotypic changes on suppressor cells. In the present study we investigated the possibility that theophylline could directly induce a suppressor activity on a lymphocyte subpopulation. We observed that a short preincubation (120 min at 37°C) with theophylline (1 mM) activates human peripheral blood lymphocytes to suppress mitogenic response of autologous cells. This activity was not evident on a T cell subpopulation depleted of theophylline-sensitive (T-sens) lymphocytes. Theophylline mediated suppressor activity is only present in the Concanavalin A stimulated cultures, thus suggesting a synergism between Concanavalin A and theophylline in the expression of non specific suppression. Moreover we observed that after a 24 hrs preincubation of lymphocytes in complete culture medium there was a complete loss of theophylline-induced suppression. Such a preincubation time also produced a decrease in the theophylline-mediated enhancement of intracellular 3',5' cyclic adenosine monophosphate levels and the impairment of E-rosette formation, suggesting that theophylline acts mainly on a 'short-lived' suppressor lymphocyte subset.

AB - It is well known that theophylline yields phenotypic changes on suppressor cells. In the present study we investigated the possibility that theophylline could directly induce a suppressor activity on a lymphocyte subpopulation. We observed that a short preincubation (120 min at 37°C) with theophylline (1 mM) activates human peripheral blood lymphocytes to suppress mitogenic response of autologous cells. This activity was not evident on a T cell subpopulation depleted of theophylline-sensitive (T-sens) lymphocytes. Theophylline mediated suppressor activity is only present in the Concanavalin A stimulated cultures, thus suggesting a synergism between Concanavalin A and theophylline in the expression of non specific suppression. Moreover we observed that after a 24 hrs preincubation of lymphocytes in complete culture medium there was a complete loss of theophylline-induced suppression. Such a preincubation time also produced a decrease in the theophylline-mediated enhancement of intracellular 3',5' cyclic adenosine monophosphate levels and the impairment of E-rosette formation, suggesting that theophylline acts mainly on a 'short-lived' suppressor lymphocyte subset.

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