Therapeutic faecal microbiota transplantation controls intestinal inflammation through IL10 secretion by immune cells

Claudia Burrello, Federica Garavaglia, Fulvia Milena Cribiù, Giulia Ercoli, Gianluca Lopez, Jacopo Troisi, Angelo Colucci, Silvia Guglietta, Sara Carloni, Simone Guglielmetti, Valentina Taverniti, Giulia Nizzoli, Silvano Bosari, Flavio Caprioli, Maria Rescigno, Federica Facciotti

Research output: Contribution to journalArticlepeer-review


Alteration of the gut microbiota has been associated with different gastrointestinal disorders. Normobiosis restoration by faecal microbiota transplantation (FMT) is considered a promising therapeutic approach, even if the mechanisms underlying its efficacy are at present largely unknown. Here we sought to elucidate the functional effects of therapeutic FMT administration during experimental colitis on innate and adaptive immune responses in the intestinal mucosa. We show that therapeutic FMT reduces colonic inflammation and initiates the restoration of intestinal homeostasis through the simultaneous activation of different immune-mediated pathways, ultimately leading to IL-10 production by innate and adaptive immune cells, including CD4+ T cells, iNKT cells and Antigen Presenting Cells (APC), and reduces the ability of dendritic cells, monocytes and macrophages to present MHCII-dependent bacterial antigens to colonic T cells. These results demonstrate the capability of FMT to therapeutically control intestinal experimental colitis and poses FMT as a valuable therapeutic option in immune-related pathologies.

Original languageEnglish
Pages (from-to)5184
JournalNature Communications
Issue number1
Publication statusPublished - Dec 5 2018


  • Adaptive Immunity
  • Animals
  • Antigen-Presenting Cells/immunology
  • CD4-Positive T-Lymphocytes/immunology
  • Colitis/genetics
  • Dendritic Cells/immunology
  • Disease Models, Animal
  • Fecal Microbiota Transplantation
  • Female
  • Gastrointestinal Microbiome
  • Humans
  • Immunity, Innate
  • Interleukin-10/genetics
  • Intestinal Mucosa/immunology
  • Macrophages/immunology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Natural Killer T-Cells/immunology


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