Therapeutic gamma-hydroxybutyric acid monitoring in plasma and urine by gas chromatography-mass spectrometry

S. D. Ferrara, L. Tedeschi, G. Frison, F. Castagna, L. Gallimberti, R. Giorgetti, G. L. Gessa, P. Palatini

Research output: Contribution to journalArticlepeer-review

Abstract

A gas chromatographic-mass spectrometric (GC-MS) method for the determination of therapeutic levels of gamma-hydroxybutyric acid (GHB) in plasma and urine samples is described. GHB is converted to its lactonic form gamma-butyrolactone (GBL) which is extracted from biological fluids after the addition of the internal standard delta-valerolactone. Final GC-MS analysis is obtained under electron impact selected ion monitoring (SIM) conditions. Mean relative recoveries of GHB from plasma and urine are 75.5% (RSD% = 2.2) and 76.4% (RSD% = 2.4), respectively. The assay is linear over a plasma GHB range of 2-200 μg ml-1 (r = 0.999) and a urine GHB range of 2-150 μg ml-1 (r = 0.998). Intra- and inter-assay relative standard deviations (n = 5) determined at 10 and 100 μg ml-1 are below 5%. The method is simple, specific and accurate, and may be applied for analytical purposes related to pharmacokinetic studies and therapeutic drug monitoring.

Original languageEnglish
Pages (from-to)483-487
Number of pages5
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume11
Issue number6
DOIs
Publication statusPublished - 1993

Keywords

  • Gamma-hydroxybutyric acid
  • GC-MS.
  • therapeutic drug monitoring

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

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