Thermodynamics of HMGB1 interaction with duplex DNA

S. Müller, M. E. Bianchi, S. Knapp

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The high mobility group protein HMGB1 is a small, highly abundant protein that binds to DNA in a non-sequence-specific manner. HMGB1 consists of 2 DNA binding domains, the HMG boxes A and B, followed by a short basic region and a continuous stretch of 30 glutamate or aspartate residues. Isothermal titration calorimetry was used to characterize the binding of HMGB1 to the double-stranded model DNAs poly(dAdT)·(dTdA) and poly(dGdC)·(dCdG). To elucidate the contribution of the different structural motifs to DNA binding, calorimetric measurements were performed comparing the single boxes A and B, the two boxes plus or minus the basic sequence stretch (ABbt and AB), and the full-length HMGB1 protein. Thermodynamically, binding of HMGB1 and all truncated constructs to duplex DNA was characterized by a positive enthalpy change at 15 °C. From the slopes of the temperature dependence of the binding enthalpies, heat capacity changes of -0.129 ± 0.02 and -0.105 ± 0.05 kcal mol-1 K-1 were determined for box A and full-length HMGB1, respectively. Significant differences in the binding characteristics were observed using full-length HMGB1, suggesting an important role for the acid tail in modulating DNA binding. Moreover, full-length HMGB1 binds differently these two DNA templates: binding to poly(dAdT)·(dTdA) was cooperative, had a larger apparent binding site size, and proceeded with a much larger unfavorable binding enthalpy than binding to poly(dGdC)·(dCdG).

Original languageEnglish
Pages (from-to)10254-10261
Number of pages8
JournalBiochemistry
Volume40
Issue number34
DOIs
Publication statusPublished - Aug 28 2001

Fingerprint

HMGB1 Protein
Thermodynamics
DNA
Enthalpy
HMG-Box Domains
High Mobility Group Proteins
Calorimetry
Nucleotide Motifs
Titration
Aspartic Acid
Specific heat
Glutamic Acid
Hot Temperature
Binding Sites
Temperature
Acids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Thermodynamics of HMGB1 interaction with duplex DNA. / Müller, S.; Bianchi, M. E.; Knapp, S.

In: Biochemistry, Vol. 40, No. 34, 28.08.2001, p. 10254-10261.

Research output: Contribution to journalArticle

Müller, S. ; Bianchi, M. E. ; Knapp, S. / Thermodynamics of HMGB1 interaction with duplex DNA. In: Biochemistry. 2001 ; Vol. 40, No. 34. pp. 10254-10261.
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