Thermosensitive eyedrops containing platelet lysate for the treatment of corneal ulcers

Giuseppina Sandri, Maria Cristina Bonferoni, Silvia Rossi, Franca Ferrari, Michela Mori, Claudia Del Fante, Cesare Perotti, Carla Caramella

Research output: Contribution to journalArticlepeer-review

Abstract

Corneal lesions cause significant pain and visual impairment and, in many cases, are unresponsive to conventional treatments. Platelet lysate (PL) is an haemoderivative rich in growth factors (GFs) that are released by platelets after freeze-thawing destruction of platelet rich plasma (PRP). The aim of the present work was to develop thermosensitive and mucoadhesive eyedrops to maintain and prolong the contact of platelet lysate (PL) with cornea ulcers. A sterile vehicle based on chondroitin sulphate sodium (CS) and hydroxypropylmethyl cellulose (HPMC) was developed. An extemporaneous loading of the vehicle with PL was performed and the obtained formulation was able to quickly thermogelify at about 32 °C and was characterized by good mucoadhesive properties. ELISA evidenced that the growth factor PDGF AB was compatible with the vehicle and stable in the formulation up to 15 days of storage at 2-8 °C. In vitro wound healing and proliferation test (performed using rabbit corneal epithelial cells (RCE)) showed that the formulation enhanced cell growth and put in evidence a synergistic effect of CS and PL in stimulating cell proliferation. The overall results indicate that PL loaded in thermosensitive and mucoadhesive eyedrops can be profitably employed to treat corneal lesions.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalInternational Journal of Pharmaceutics
Volume426
Issue number1-2
DOIs
Publication statusPublished - Apr 15 2012

Keywords

  • Cornea
  • Growth factors
  • Mucoadhesion
  • Platelet lysate
  • Thermogelling
  • Wound healing

ASJC Scopus subject areas

  • Pharmaceutical Science

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